Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23470 | 5' | -57.3 | NC_005259.1 | + | 19841 | 0.77 | 0.150016 |
Target: 5'- cGGCUGCU-GCCGCGCUCgguguggcgCUCGCg -3' miRNA: 3'- uUCGACGAgUGGCGCGAGaa-------GAGCGg -5' |
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23470 | 5' | -57.3 | NC_005259.1 | + | 29143 | 0.74 | 0.2294 |
Target: 5'- -cGCcucGCUCACCGCGCUCggCUgGaCCg -3' miRNA: 3'- uuCGa--CGAGUGGCGCGAGaaGAgC-GG- -5' |
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23470 | 5' | -57.3 | NC_005259.1 | + | 45550 | 0.71 | 0.333446 |
Target: 5'- cGGGUUGCUCGCCGgGCcaccCUUGCCc -3' miRNA: 3'- -UUCGACGAGUGGCgCGagaaGAGCGG- -5' |
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23470 | 5' | -57.3 | NC_005259.1 | + | 13470 | 0.71 | 0.347208 |
Target: 5'- -uGgUGCUCACCGCGCugcccgacgaugucUCgguccCUCGCCc -3' miRNA: 3'- uuCgACGAGUGGCGCG--------------AGaa---GAGCGG- -5' |
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23470 | 5' | -57.3 | NC_005259.1 | + | 33129 | 0.71 | 0.366465 |
Target: 5'- cGGCaGC-CGCCGCGCgugaUCUUGCCg -3' miRNA: 3'- uUCGaCGaGUGGCGCGaga-AGAGCGG- -5' |
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23470 | 5' | -57.3 | NC_005259.1 | + | 31563 | 0.7 | 0.401668 |
Target: 5'- -uGCcGCUgCACCGCGC-CUgaUCgUCGCCa -3' miRNA: 3'- uuCGaCGA-GUGGCGCGaGA--AG-AGCGG- -5' |
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23470 | 5' | -57.3 | NC_005259.1 | + | 18253 | 0.69 | 0.423795 |
Target: 5'- cGAGCUGCcCGCCGCGC-CUgaaccugagacgaccUCgaCGCCc -3' miRNA: 3'- -UUCGACGaGUGGCGCGaGA---------------AGa-GCGG- -5' |
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23470 | 5' | -57.3 | NC_005259.1 | + | 2211 | 0.69 | 0.458311 |
Target: 5'- -uGCUGCgCACCgaGCGCcCggcCUCGCCa -3' miRNA: 3'- uuCGACGaGUGG--CGCGaGaa-GAGCGG- -5' |
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23470 | 5' | -57.3 | NC_005259.1 | + | 5508 | 0.68 | 0.474127 |
Target: 5'- cGAGCUacGCgCGCCGCGCccgcaagcuguggCUgcUCUCGCCc -3' miRNA: 3'- -UUCGA--CGaGUGGCGCGa------------GA--AGAGCGG- -5' |
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23470 | 5' | -57.3 | NC_005259.1 | + | 58823 | 0.68 | 0.495291 |
Target: 5'- -cGCUGCUCggcacccucGCCGCcCUCggucucacccucgggCUCGCCg -3' miRNA: 3'- uuCGACGAG---------UGGCGcGAGaa-------------GAGCGG- -5' |
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23470 | 5' | -57.3 | NC_005259.1 | + | 5202 | 0.68 | 0.529372 |
Target: 5'- -cGCcaaGCUCACCGCGCUCgccgaGCa -3' miRNA: 3'- uuCGa--CGAGUGGCGCGAGaagagCGg -5' |
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23470 | 5' | -57.3 | NC_005259.1 | + | 8911 | 0.67 | 0.561078 |
Target: 5'- -uGCUGaCggGCCGCGUUCUgggCUCGUUu -3' miRNA: 3'- uuCGAC-GagUGGCGCGAGAa--GAGCGG- -5' |
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23470 | 5' | -57.3 | NC_005259.1 | + | 48015 | 0.67 | 0.593281 |
Target: 5'- uGAGCUGCUgCACCGCGCcCacgaUgUUGaCCa -3' miRNA: 3'- -UUCGACGA-GUGGCGCGaGa---AgAGC-GG- -5' |
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23470 | 5' | -57.3 | NC_005259.1 | + | 50697 | 0.67 | 0.582504 |
Target: 5'- -cGCUGCUCGucgUCGCGCUUga---GCCg -3' miRNA: 3'- uuCGACGAGU---GGCGCGAGaagagCGG- -5' |
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23470 | 5' | -57.3 | NC_005259.1 | + | 31810 | 0.67 | 0.582504 |
Target: 5'- cGAGCUcGaccgacccCACCGCGCccgcgucgaUCUUCUCGCg -3' miRNA: 3'- -UUCGA-Cga------GUGGCGCG---------AGAAGAGCGg -5' |
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23470 | 5' | -57.3 | NC_005259.1 | + | 52026 | 0.67 | 0.582504 |
Target: 5'- cAGGCcGCcgCACUGCGCgCUg-UCGCCg -3' miRNA: 3'- -UUCGaCGa-GUGGCGCGaGAagAGCGG- -5' |
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23470 | 5' | -57.3 | NC_005259.1 | + | 1364 | 0.67 | 0.582504 |
Target: 5'- uGGGCcGCUCGCCGCGC-CgcC-CGCg -3' miRNA: 3'- -UUCGaCGAGUGGCGCGaGaaGaGCGg -5' |
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23470 | 5' | -57.3 | NC_005259.1 | + | 63646 | 0.67 | 0.571767 |
Target: 5'- cGAGCUGCUCGCUG-GC-CgggaCGCCg -3' miRNA: 3'- -UUCGACGAGUGGCgCGaGaagaGCGG- -5' |
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23470 | 5' | -57.3 | NC_005259.1 | + | 48603 | 0.67 | 0.571767 |
Target: 5'- cGAGCgcggGCgcuacCGCCGUGCgcaCUgcCUCGCCg -3' miRNA: 3'- -UUCGa---CGa----GUGGCGCGa--GAa-GAGCGG- -5' |
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23470 | 5' | -57.3 | NC_005259.1 | + | 14720 | 0.67 | 0.550445 |
Target: 5'- ----aGCaCGCCGCGCUCg--UCGCCg -3' miRNA: 3'- uucgaCGaGUGGCGCGAGaagAGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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