Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23471 | 3' | -60.3 | NC_005259.1 | + | 16431 | 0.72 | 0.195877 |
Target: 5'- cGUCGAGcGGCccaaaccgGGCACCGuCGGCAUCGg -3' miRNA: 3'- -CAGCUC-CCG--------CCGUGGCcGCUGUAGUa -5' |
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23471 | 3' | -60.3 | NC_005259.1 | + | 58727 | 0.7 | 0.264933 |
Target: 5'- -cCGGGGGUgucguuggcaccGGCACCGGCGAaguUCGg -3' miRNA: 3'- caGCUCCCG------------CCGUGGCCGCUgu-AGUa -5' |
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23471 | 3' | -60.3 | NC_005259.1 | + | 53010 | 0.7 | 0.285028 |
Target: 5'- cUCGAcuGGGC-GCugCGGUGGCGUCGa -3' miRNA: 3'- cAGCU--CCCGcCGugGCCGCUGUAGUa -5' |
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23471 | 3' | -60.3 | NC_005259.1 | + | 27139 | 0.69 | 0.306308 |
Target: 5'- uUUGAGGGCGGCugCGGUGuuGg--- -3' miRNA: 3'- cAGCUCCCGCCGugGCCGCugUagua -5' |
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23471 | 3' | -60.3 | NC_005259.1 | + | 57311 | 0.69 | 0.313667 |
Target: 5'- aUCGAGcaccGGCGGUGUCGGUGugGUCAUg -3' miRNA: 3'- cAGCUC----CCGCCGUGGCCGCugUAGUA- -5' |
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23471 | 3' | -60.3 | NC_005259.1 | + | 64024 | 0.69 | 0.328783 |
Target: 5'- -cCGAGGcGCGGCgagGCCGGUGACcggAUCu- -3' miRNA: 3'- caGCUCC-CGCCG---UGGCCGCUG---UAGua -5' |
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23471 | 3' | -60.3 | NC_005259.1 | + | 17354 | 0.69 | 0.336541 |
Target: 5'- cGUCGAGGucggcggcuCGGCGCUGGuCGugGUCGa -3' miRNA: 3'- -CAGCUCCc--------GCCGUGGCC-GCugUAGUa -5' |
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23471 | 3' | -60.3 | NC_005259.1 | + | 59306 | 0.69 | 0.344431 |
Target: 5'- uGUCGu-GGCGGCAgCGGCGAgcUCAg -3' miRNA: 3'- -CAGCucCCGCCGUgGCCGCUguAGUa -5' |
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23471 | 3' | -60.3 | NC_005259.1 | + | 63842 | 0.68 | 0.377306 |
Target: 5'- uUCGAGGcaaGCGcGUACCGGCGAgGUgGc -3' miRNA: 3'- cAGCUCC---CGC-CGUGGCCGCUgUAgUa -5' |
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23471 | 3' | -60.3 | NC_005259.1 | + | 61732 | 0.68 | 0.385849 |
Target: 5'- uGUCGAGcaGGUGuGCGuCCGGCGAgAUCu- -3' miRNA: 3'- -CAGCUC--CCGC-CGU-GGCCGCUgUAGua -5' |
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23471 | 3' | -60.3 | NC_005259.1 | + | 51101 | 0.68 | 0.385849 |
Target: 5'- -aCGAGGGCGGgAucuguCCGGCaGGcCAUCAg -3' miRNA: 3'- caGCUCCCGCCgU-----GGCCG-CU-GUAGUa -5' |
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23471 | 3' | -60.3 | NC_005259.1 | + | 9049 | 0.68 | 0.385849 |
Target: 5'- -gCG-GGGCGGUaACCGGCucgGugGUCAUg -3' miRNA: 3'- caGCuCCCGCCG-UGGCCG---CugUAGUA- -5' |
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23471 | 3' | -60.3 | NC_005259.1 | + | 5079 | 0.68 | 0.360608 |
Target: 5'- gGUCGAcGGGcCGGUgucACCGGCGAUcugCGUa -3' miRNA: 3'- -CAGCU-CCC-GCCG---UGGCCGCUGua-GUA- -5' |
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23471 | 3' | -60.3 | NC_005259.1 | + | 38627 | 0.67 | 0.403312 |
Target: 5'- aUCGAGGGCGGUgacGCCgaguuGGCGAacUCGc -3' miRNA: 3'- cAGCUCCCGCCG---UGG-----CCGCUguAGUa -5' |
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23471 | 3' | -60.3 | NC_005259.1 | + | 9339 | 0.67 | 0.412229 |
Target: 5'- uGUCGAGGuCGGCACCGaUGAcCGUCu- -3' miRNA: 3'- -CAGCUCCcGCCGUGGCcGCU-GUAGua -5' |
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23471 | 3' | -60.3 | NC_005259.1 | + | 10339 | 0.67 | 0.412229 |
Target: 5'- -aCGGGcucGGCGGCAgCGGgcuCGACAUCGg -3' miRNA: 3'- caGCUC---CCGCCGUgGCC---GCUGUAGUa -5' |
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23471 | 3' | -60.3 | NC_005259.1 | + | 61579 | 0.67 | 0.429503 |
Target: 5'- aGUUGAGGaucucauGCGGUggGCCGGUGGgGUCGg -3' miRNA: 3'- -CAGCUCC-------CGCCG--UGGCCGCUgUAGUa -5' |
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23471 | 3' | -60.3 | NC_005259.1 | + | 1233 | 0.66 | 0.48768 |
Target: 5'- -aCGAGGaCGGCugcaagACCGGCcucGGCAUCAc -3' miRNA: 3'- caGCUCCcGCCG------UGGCCG---CUGUAGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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