Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23472 | 5' | -54.7 | NC_005259.1 | + | 40190 | 0.66 | 0.807441 |
Target: 5'- aGCCGUugaccUCCGagagucauguuCUGCUGCUUgCCGUUGg -3' miRNA: 3'- cCGGUA-----GGGC-----------GACGAUGAA-GGCAACa -5' |
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23472 | 5' | -54.7 | NC_005259.1 | + | 45884 | 0.68 | 0.684494 |
Target: 5'- aGGCCA-CCCGCUGUgUugUUCgGUcGg -3' miRNA: 3'- -CCGGUaGGGCGACG-AugAAGgCAaCa -5' |
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23472 | 5' | -54.7 | NC_005259.1 | + | 62630 | 0.7 | 0.543504 |
Target: 5'- uGGCCAgcaCCGCauUGCguaGCUUCuCGUUGUc -3' miRNA: 3'- -CCGGUag-GGCG--ACGa--UGAAG-GCAACA- -5' |
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23472 | 5' | -54.7 | NC_005259.1 | + | 36854 | 0.7 | 0.543504 |
Target: 5'- cGCCG-CCCGCUGCg----CCGUUGc -3' miRNA: 3'- cCGGUaGGGCGACGaugaaGGCAACa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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