Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23473 | 3' | -52.2 | NC_005259.1 | + | 62518 | 0.66 | 0.935524 |
Target: 5'- -----aGCGGUcaGCAcUUCCUCGGCCu -3' miRNA: 3'- ggaguaUGCCGagCGU-AAGGAGCUGG- -5' |
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23473 | 3' | -52.2 | NC_005259.1 | + | 49212 | 0.66 | 0.935524 |
Target: 5'- uCCgUUGUGCGGgguuuaCUCGUcgUCggaCUCGACCg -3' miRNA: 3'- -GG-AGUAUGCC------GAGCGuaAG---GAGCUGG- -5' |
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23473 | 3' | -52.2 | NC_005259.1 | + | 45434 | 0.66 | 0.930144 |
Target: 5'- gCCUCGUA-GGC-CGgAUcucggUCCUUGAUCg -3' miRNA: 3'- -GGAGUAUgCCGaGCgUA-----AGGAGCUGG- -5' |
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23473 | 3' | -52.2 | NC_005259.1 | + | 62105 | 0.66 | 0.928477 |
Target: 5'- aUCUCGU-CGcGCUCGUAgucagUCaccgaggugaucguCUCGACCa -3' miRNA: 3'- -GGAGUAuGC-CGAGCGUa----AG--------------GAGCUGG- -5' |
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23473 | 3' | -52.2 | NC_005259.1 | + | 67346 | 0.66 | 0.924492 |
Target: 5'- aCCaCGUAC-GCUCGCcgaCCUCGcGCCg -3' miRNA: 3'- -GGaGUAUGcCGAGCGuaaGGAGC-UGG- -5' |
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23473 | 3' | -52.2 | NC_005259.1 | + | 8368 | 0.66 | 0.918568 |
Target: 5'- gCUCGgcgGCGGcCUCgGCAUcggcgagagCCUCGGCg -3' miRNA: 3'- gGAGUa--UGCC-GAG-CGUAa--------GGAGCUGg -5' |
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23473 | 3' | -52.2 | NC_005259.1 | + | 41832 | 0.66 | 0.912373 |
Target: 5'- aCC-CAUuggauCGGCUCGa---CCUCGACg -3' miRNA: 3'- -GGaGUAu----GCCGAGCguaaGGAGCUGg -5' |
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23473 | 3' | -52.2 | NC_005259.1 | + | 24722 | 0.67 | 0.899863 |
Target: 5'- gCCUCGgcgaucacgauugaUGCGGUguggggugccgaggCGCucaucgaaUCCUCGACCa -3' miRNA: 3'- -GGAGU--------------AUGCCGa-------------GCGua------AGGAGCUGG- -5' |
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23473 | 3' | -52.2 | NC_005259.1 | + | 29806 | 0.67 | 0.892183 |
Target: 5'- aCCUCAgucgagACGaGgUCGCuaagcgCCUCGGCa -3' miRNA: 3'- -GGAGUa-----UGC-CgAGCGuaa---GGAGCUGg -5' |
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23473 | 3' | -52.2 | NC_005259.1 | + | 4114 | 0.67 | 0.892183 |
Target: 5'- aCCUCG-GCGGcCUCGaCGUggCCaaGACCg -3' miRNA: 3'- -GGAGUaUGCC-GAGC-GUAa-GGagCUGG- -5' |
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23473 | 3' | -52.2 | NC_005259.1 | + | 23527 | 0.67 | 0.884929 |
Target: 5'- aUCUCG-ACGGUgaaaaucagcCGCcg-CCUCGACCg -3' miRNA: 3'- -GGAGUaUGCCGa---------GCGuaaGGAGCUGG- -5' |
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23473 | 3' | -52.2 | NC_005259.1 | + | 52208 | 0.67 | 0.884929 |
Target: 5'- gCUCAUG-GGCacCGCGUUCCgCGcCCg -3' miRNA: 3'- gGAGUAUgCCGa-GCGUAAGGaGCuGG- -5' |
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23473 | 3' | -52.2 | NC_005259.1 | + | 29211 | 0.67 | 0.884929 |
Target: 5'- cCCcCGcAUGGUUCGCcagCCUCGGCg -3' miRNA: 3'- -GGaGUaUGCCGAGCGuaaGGAGCUGg -5' |
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23473 | 3' | -52.2 | NC_005259.1 | + | 58856 | 0.67 | 0.877421 |
Target: 5'- cCCUCG---GGCUCGCcgUCgUCGucgucGCCu -3' miRNA: 3'- -GGAGUaugCCGAGCGuaAGgAGC-----UGG- -5' |
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23473 | 3' | -52.2 | NC_005259.1 | + | 50939 | 0.67 | 0.877421 |
Target: 5'- --aCGaACGGCUCGUugcaUCCUUGGCUc -3' miRNA: 3'- ggaGUaUGCCGAGCGua--AGGAGCUGG- -5' |
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23473 | 3' | -52.2 | NC_005259.1 | + | 54990 | 0.67 | 0.874347 |
Target: 5'- aCCUCAUcgaucuugugcuCGGCUCGCuuggcggucgUCUCGGCa -3' miRNA: 3'- -GGAGUAu-----------GCCGAGCGuaa-------GGAGCUGg -5' |
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23473 | 3' | -52.2 | NC_005259.1 | + | 55704 | 0.68 | 0.861663 |
Target: 5'- gCCUCGUugAUGGC-CGCGagcaCCUCGcCCu -3' miRNA: 3'- -GGAGUA--UGCCGaGCGUaa--GGAGCuGG- -5' |
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23473 | 3' | -52.2 | NC_005259.1 | + | 28829 | 0.68 | 0.836281 |
Target: 5'- -aUCA-ACGGCacCGCGUUCUacaaagUCGACCg -3' miRNA: 3'- ggAGUaUGCCGa-GCGUAAGG------AGCUGG- -5' |
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23473 | 3' | -52.2 | NC_005259.1 | + | 10579 | 0.68 | 0.836281 |
Target: 5'- gCCUCG-ACGGCgaaaUCGUcgUUgUUGACCu -3' miRNA: 3'- -GGAGUaUGCCG----AGCGuaAGgAGCUGG- -5' |
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23473 | 3' | -52.2 | NC_005259.1 | + | 50012 | 0.68 | 0.827386 |
Target: 5'- cCCUCGUGCacgGGCgcugCGUAaUCCgcugUGGCCu -3' miRNA: 3'- -GGAGUAUG---CCGa---GCGUaAGGa---GCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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