Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23473 | 5' | -51.3 | NC_005259.1 | + | 28006 | 0.74 | 0.546086 |
Target: 5'- gCCGGggcGGGGucGCCGggGUUGCgCGc -3' miRNA: 3'- -GGCCaa-CUCCuuUGGCuuCAACG-GCa -5' |
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23473 | 5' | -51.3 | NC_005259.1 | + | 46529 | 0.72 | 0.666739 |
Target: 5'- gCCGGUcGGGGAucGCCGAgaucuGGUcGCCGa -3' miRNA: 3'- -GGCCAaCUCCUu-UGGCU-----UCAaCGGCa -5' |
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23473 | 5' | -51.3 | NC_005259.1 | + | 54720 | 0.71 | 0.710326 |
Target: 5'- cCUGGUacAGGAcauAGCCGAGGcUGCCGa -3' miRNA: 3'- -GGCCAacUCCU---UUGGCUUCaACGGCa -5' |
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23473 | 5' | -51.3 | NC_005259.1 | + | 57223 | 0.71 | 0.742244 |
Target: 5'- aCCGGUUGucGGggUCGGAgauccacgucGUUGCCGg -3' miRNA: 3'- -GGCCAACucCUuuGGCUU----------CAACGGCa -5' |
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23473 | 5' | -51.3 | NC_005259.1 | + | 18839 | 0.7 | 0.773154 |
Target: 5'- cCCGG-UGAGGGucucGCCGG---UGCCGUg -3' miRNA: 3'- -GGCCaACUCCUu---UGGCUucaACGGCA- -5' |
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23473 | 5' | -51.3 | NC_005259.1 | + | 38432 | 0.69 | 0.821593 |
Target: 5'- uUGGUcGAGucGAGACCGAAaaUGCCGUg -3' miRNA: 3'- gGCCAaCUC--CUUUGGCUUcaACGGCA- -5' |
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23473 | 5' | -51.3 | NC_005259.1 | + | 26809 | 0.69 | 0.830721 |
Target: 5'- cCCGGUUGGccGGu-ACCGgcGguggUGCCGg -3' miRNA: 3'- -GGCCAACU--CCuuUGGCuuCa---ACGGCa -5' |
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23473 | 5' | -51.3 | NC_005259.1 | + | 67249 | 0.68 | 0.865041 |
Target: 5'- -gGGUUGAGcGggGCCucGGgcaGCCGUg -3' miRNA: 3'- ggCCAACUC-CuuUGGcuUCaa-CGGCA- -5' |
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23473 | 5' | -51.3 | NC_005259.1 | + | 64421 | 0.67 | 0.902419 |
Target: 5'- gCCG--UGAGGGAucGCCGAcgAGcUGCCGg -3' miRNA: 3'- -GGCcaACUCCUU--UGGCU--UCaACGGCa -5' |
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23473 | 5' | -51.3 | NC_005259.1 | + | 42360 | 0.67 | 0.902419 |
Target: 5'- gCCGGUagacaugaucguUGAGGAAGgCGGGcagGCCGg -3' miRNA: 3'- -GGCCA------------ACUCCUUUgGCUUcaaCGGCa -5' |
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23473 | 5' | -51.3 | NC_005259.1 | + | 794 | 0.67 | 0.909099 |
Target: 5'- cUCGGUUG-GGugGCC-AAGggGCCGg -3' miRNA: 3'- -GGCCAACuCCuuUGGcUUCaaCGGCa -5' |
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23473 | 5' | -51.3 | NC_005259.1 | + | 43959 | 0.67 | 0.921632 |
Target: 5'- aUGGgcGGGGccgccgccuGACCGAGGgcggUGCCGa -3' miRNA: 3'- gGCCaaCUCCu--------UUGGCUUCa---ACGGCa -5' |
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23473 | 5' | -51.3 | NC_005259.1 | + | 43068 | 0.67 | 0.927482 |
Target: 5'- gUCGG-UGAGGAAcgCGAGcccGUUGCCGc -3' miRNA: 3'- -GGCCaACUCCUUugGCUU---CAACGGCa -5' |
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23473 | 5' | -51.3 | NC_005259.1 | + | 31188 | 0.66 | 0.938349 |
Target: 5'- gUCGGc-GAGGAugccGCCGAAGaucgggaUGCCGUu -3' miRNA: 3'- -GGCCaaCUCCUu---UGGCUUCa------ACGGCA- -5' |
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23473 | 5' | -51.3 | NC_005259.1 | + | 31718 | 0.66 | 0.943367 |
Target: 5'- gCCGGUcuucucUGAGGuGACCGcgccUGCCGc -3' miRNA: 3'- -GGCCA------ACUCCuUUGGCuucaACGGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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