Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23474 | 5' | -56.9 | NC_005259.1 | + | 55222 | 0.68 | 0.550836 |
Target: 5'- aCG-UGAGCAuCGUGUucGaUGCCCACGAc -3' miRNA: 3'- cGCaACUCGU-GCACG--CcACGGGUGCU- -5' |
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23474 | 5' | -56.9 | NC_005259.1 | + | 59136 | 0.72 | 0.36929 |
Target: 5'- aGCGUcGAGCuCGgcaGCGGUGCCgGgGAg -3' miRNA: 3'- -CGCAaCUCGuGCa--CGCCACGGgUgCU- -5' |
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23474 | 5' | -56.9 | NC_005259.1 | + | 62003 | 0.7 | 0.469755 |
Target: 5'- ---aUGGGCAcCGUGCGGUGuaCCCGCu- -3' miRNA: 3'- cgcaACUCGU-GCACGCCAC--GGGUGcu -5' |
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23474 | 5' | -56.9 | NC_005259.1 | + | 67441 | 0.68 | 0.561307 |
Target: 5'- cGC-UUGAGCGCGUcGgGGaUGCCCuuuucgGCGAu -3' miRNA: 3'- -CGcAACUCGUGCA-CgCC-ACGGG------UGCU- -5' |
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23474 | 5' | -56.9 | NC_005259.1 | + | 68704 | 0.7 | 0.440926 |
Target: 5'- gGCGggcAGCGCGU-CGGUGCCCGaGAa -3' miRNA: 3'- -CGCaacUCGUGCAcGCCACGGGUgCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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