Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23479 | 3' | -61.7 | NC_005259.1 | + | 63671 | 0.7 | 0.251885 |
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Target: 5'- gGACaCCUCGGgCaCCGGCGcgccgggGAucUGCGCCu -3' miRNA: 3'- -UUG-GGAGCCgG-GGCCGCa------CU--ACGUGG- -5' |
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| 23479 | 3' | -61.7 | NC_005259.1 | + | 67760 | 0.7 | 0.25127 |
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Target: 5'- cGCCC-CGGCCaCCGGCcaccgaccacgcgGUGAccuuguugGCACCg -3' miRNA: 3'- uUGGGaGCCGG-GGCCG-------------CACUa-------CGUGG- -5' |
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| 23479 | 3' | -61.7 | NC_005259.1 | + | 31175 | 0.71 | 0.222617 |
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Target: 5'- cGCCCUUGaGCCaguCGGCGagGAUGcCGCCg -3' miRNA: 3'- uUGGGAGC-CGGg--GCCGCa-CUAC-GUGG- -5' |
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| 23479 | 3' | -61.7 | NC_005259.1 | + | 8764 | 0.71 | 0.20133 |
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Target: 5'- aGGCCCUUGGCCagcgCGGCgGUGAUGaGCUc -3' miRNA: 3'- -UUGGGAGCCGGg---GCCG-CACUACgUGG- -5' |
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| 23479 | 3' | -61.7 | NC_005259.1 | + | 64804 | 0.72 | 0.186547 |
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Target: 5'- uGugCCUCGGCCUCGGCGgcaaugagcgugUGGUGacggACCc -3' miRNA: 3'- -UugGGAGCCGGGGCCGC------------ACUACg---UGG- -5' |
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| 23479 | 3' | -61.7 | NC_005259.1 | + | 42640 | 0.72 | 0.172728 |
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Target: 5'- aGGCaCCUCGGCCCCGaUGUGG-GCgGCCa -3' miRNA: 3'- -UUG-GGAGCCGGGGCcGCACUaCG-UGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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