Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23479 | 5' | -50.9 | NC_005259.1 | + | 46754 | 0.66 | 0.958397 |
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Target: 5'- uGGcUGCGcCGACaccugcGCCGACAGCc -3' miRNA: 3'- uCUuACGCaGCUGccuu--UGGCUGUCG- -5' |
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| 23479 | 5' | -50.9 | NC_005259.1 | + | 27372 | 0.66 | 0.958397 |
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Target: 5'- -uGGUGCc-CGgcACGGuGACCGGCGGCg -3' miRNA: 3'- ucUUACGcaGC--UGCCuUUGGCUGUCG- -5' |
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| 23479 | 5' | -50.9 | NC_005259.1 | + | 3122 | 0.66 | 0.958397 |
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Target: 5'- ----cGCGgcacCGugGGcAGCgGACGGCa -3' miRNA: 3'- ucuuaCGCa---GCugCCuUUGgCUGUCG- -5' |
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| 23479 | 5' | -50.9 | NC_005259.1 | + | 43398 | 0.66 | 0.959969 |
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Target: 5'- gAGGAUGCGaauacguuccUCGGCGGcgguuuccucgcucGACUGGCuGCg -3' miRNA: 3'- -UCUUACGC----------AGCUGCCu-------------UUGGCUGuCG- -5' |
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| 23479 | 5' | -50.9 | NC_005259.1 | + | 24739 | 0.66 | 0.962249 |
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Target: 5'- -uGAUGCGgUGugGGguGCCGA-GGCg -3' miRNA: 3'- ucUUACGCaGCugCCuuUGGCUgUCG- -5' |
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| 23479 | 5' | -50.9 | NC_005259.1 | + | 11293 | 0.66 | 0.962249 |
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Target: 5'- gAGGAcG-GUCGACGGGcgugaGGCCGagacgggcgcGCAGCu -3' miRNA: 3'- -UCUUaCgCAGCUGCCU-----UUGGC----------UGUCG- -5' |
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| 23479 | 5' | -50.9 | NC_005259.1 | + | 33903 | 0.66 | 0.962249 |
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Target: 5'- ----cGCG-CGACGGGccgaGACCGGCcacggugcgAGCa -3' miRNA: 3'- ucuuaCGCaGCUGCCU----UUGGCUG---------UCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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