Results 41 - 47 of 47 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23479 | 5' | -50.9 | NC_005259.1 | + | 61603 | 0.67 | 0.923882 |
Target: 5'- cGGug-GgGUCGGCGGGgaacagcaGACCGGCAccGCc -3' miRNA: 3'- -UCuuaCgCAGCUGCCU--------UUGGCUGU--CG- -5' |
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23479 | 5' | -50.9 | NC_005259.1 | + | 61740 | 0.72 | 0.691947 |
Target: 5'- aGGuGUGCGUcCGGCGaGAucuGugCGGCGGCa -3' miRNA: 3'- -UCuUACGCA-GCUGC-CU---UugGCUGUCG- -5' |
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23479 | 5' | -50.9 | NC_005259.1 | + | 62724 | 0.66 | 0.940327 |
Target: 5'- gGGGAUcucGC-UCGAcCGGA--CCGGCGGCa -3' miRNA: 3'- -UCUUA---CGcAGCU-GCCUuuGGCUGUCG- -5' |
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23479 | 5' | -50.9 | NC_005259.1 | + | 63549 | 0.69 | 0.867912 |
Target: 5'- -aGGUGC-UCGGCGGccACCGAC-GCg -3' miRNA: 3'- ucUUACGcAGCUGCCuuUGGCUGuCG- -5' |
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23479 | 5' | -50.9 | NC_005259.1 | + | 64946 | 0.66 | 0.957997 |
Target: 5'- gAGggUGgccggguUGUCGAUGGucauGCaCGGCAGUc -3' miRNA: 3'- -UCuuAC-------GCAGCUGCCuu--UG-GCUGUCG- -5' |
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23479 | 5' | -50.9 | NC_005259.1 | + | 65115 | 0.68 | 0.883504 |
Target: 5'- -cAGUGCGUCaccguCGGcgcACCGGCGGCc -3' miRNA: 3'- ucUUACGCAGcu---GCCuu-UGGCUGUCG- -5' |
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23479 | 5' | -50.9 | NC_005259.1 | + | 67211 | 0.69 | 0.842683 |
Target: 5'- ----cGCuGUCGGCGGGGucgauGCCGACcacGGCg -3' miRNA: 3'- ucuuaCG-CAGCUGCCUU-----UGGCUG---UCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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