Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23479 | 5' | -50.9 | NC_005259.1 | + | 7880 | 0.66 | 0.958397 |
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Target: 5'- cGAGUGCuUgGAcuCGGGcagccGCCGAUAGCg -3' miRNA: 3'- uCUUACGcAgCU--GCCUu----UGGCUGUCG- -5' |
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| 23479 | 5' | -50.9 | NC_005259.1 | + | 6695 | 0.72 | 0.691947 |
|
Target: 5'- aAGAGUGgCG-CGACacGAucAACCGGCAGCa -3' miRNA: 3'- -UCUUAC-GCaGCUGc-CU--UUGGCUGUCG- -5' |
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| 23479 | 5' | -50.9 | NC_005259.1 | + | 5072 | 0.66 | 0.958397 |
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Target: 5'- uGGGccGgGUCGACGG--GCCGGugucacCGGCg -3' miRNA: 3'- -UCUuaCgCAGCUGCCuuUGGCU------GUCG- -5' |
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| 23479 | 5' | -50.9 | NC_005259.1 | + | 4118 | 0.66 | 0.949903 |
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Target: 5'- cGGcgGCcUCGACGuGGccaaGACCGuCGGCg -3' miRNA: 3'- uCUuaCGcAGCUGC-CU----UUGGCuGUCG- -5' |
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| 23479 | 5' | -50.9 | NC_005259.1 | + | 3311 | 0.77 | 0.416281 |
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Target: 5'- cGAGcgcUGCGUCGACGuGAGcGCCGgGCAGCu -3' miRNA: 3'- uCUU---ACGCAGCUGC-CUU-UGGC-UGUCG- -5' |
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| 23479 | 5' | -50.9 | NC_005259.1 | + | 3122 | 0.66 | 0.958397 |
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Target: 5'- ----cGCGgcacCGugGGcAGCgGACGGCa -3' miRNA: 3'- ucuuaCGCa---GCugCCuUUGgCUGUCG- -5' |
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| 23479 | 5' | -50.9 | NC_005259.1 | + | 3065 | 0.67 | 0.911521 |
|
Target: 5'- -----cCGUCGACGGGccgaGGCCGACAa- -3' miRNA: 3'- ucuuacGCAGCUGCCU----UUGGCUGUcg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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