Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2348 | 5' | -57.1 | NC_001416.1 | + | 46238 | 0.66 | 0.628451 |
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Target: 5'- aCCgCCUCCGGCGUGgaUAaugcagccuccCCcCGAc- -3' miRNA: 3'- -GGgGGAGGCCGCACgaAU-----------GGuGCUuc -5' |
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| 2348 | 5' | -57.1 | NC_001416.1 | + | 2363 | 0.66 | 0.595876 |
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Target: 5'- uCCCCUUCCGGCGUcaGUgUGaaGCGGu- -3' miRNA: 3'- -GGGGGAGGCCGCA--CGaAUggUGCUuc -5' |
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| 2348 | 5' | -57.1 | NC_001416.1 | + | 9562 | 0.67 | 0.542291 |
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Target: 5'- aCgCCCUCCGGC-UGgaagGCCACGGu- -3' miRNA: 3'- -GgGGGAGGCCGcACgaa-UGGUGCUuc -5' |
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| 2348 | 5' | -57.1 | NC_001416.1 | + | 10158 | 0.67 | 0.521283 |
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Target: 5'- aCCCgaCUGGCGUGCcaugcUUGCCGgGAu- -3' miRNA: 3'- gGGGgaGGCCGCACG-----AAUGGUgCUuc -5' |
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| 2348 | 5' | -57.1 | NC_001416.1 | + | 14776 | 0.68 | 0.469288 |
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Target: 5'- aCUCCCUCUGGCGUcaccgggcauggcGCgcaucUGCCuuuACGggGa -3' miRNA: 3'- -GGGGGAGGCCGCA-------------CGa----AUGG---UGCuuC- -5' |
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| 2348 | 5' | -57.1 | NC_001416.1 | + | 15777 | 0.69 | 0.403465 |
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Target: 5'- aUCCCUCCGGCGgagucugcuccUGCUcACCAgccCGGAa -3' miRNA: 3'- gGGGGAGGCCGC-----------ACGAaUGGU---GCUUc -5' |
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| 2348 | 5' | -57.1 | NC_001416.1 | + | 1506 | 0.73 | 0.25515 |
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Target: 5'- aCCCCUCCaGCGUGUUUuaucuCUGCGAGc -3' miRNA: 3'- gGGGGAGGcCGCACGAAu----GGUGCUUc -5' |
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| 2348 | 5' | -57.1 | NC_001416.1 | + | 14258 | 1.09 | 0.000628 |
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Target: 5'- uCCCCCUCCGGCGUGCUUACCACGAAGc -3' miRNA: 3'- -GGGGGAGGCCGCACGAAUGGUGCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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