Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23481 | 5' | -59.7 | NC_005259.1 | + | 50008 | 0.66 | 0.521711 |
Target: 5'- gCUGcCCUCGUGC----ACGGGCGCu-- -3' miRNA: 3'- -GAC-GGAGCGCGaccaUGCCCGCGuua -5' |
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23481 | 5' | -59.7 | NC_005259.1 | + | 17195 | 0.66 | 0.49137 |
Target: 5'- -aGCCgCGCGUgaggcgGGUAgacgggacUGGGCGCGAg -3' miRNA: 3'- gaCGGaGCGCGa-----CCAU--------GCCCGCGUUa -5' |
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23481 | 5' | -59.7 | NC_005259.1 | + | 50405 | 0.66 | 0.49137 |
Target: 5'- -cGCCgagCGCGCcGGUGCcuugaccggcucGGGCaGCGGUg -3' miRNA: 3'- gaCGGa--GCGCGaCCAUG------------CCCG-CGUUA- -5' |
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23481 | 5' | -59.7 | NC_005259.1 | + | 9914 | 0.67 | 0.471603 |
Target: 5'- -gGcCCUCGCGCUGGUcauuGCGGuugaacacguuGCGCc-- -3' miRNA: 3'- gaC-GGAGCGCGACCA----UGCC-----------CGCGuua -5' |
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23481 | 5' | -59.7 | NC_005259.1 | + | 24333 | 0.67 | 0.471603 |
Target: 5'- -gGCCUcaCGCGCUGGccuaccgcCGGGUGCu-- -3' miRNA: 3'- gaCGGA--GCGCGACCau------GCCCGCGuua -5' |
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23481 | 5' | -59.7 | NC_005259.1 | + | 64442 | 0.67 | 0.452247 |
Target: 5'- gCUGCCgggGCGCUGcccUugGGGCGgCGAa -3' miRNA: 3'- -GACGGag-CGCGACc--AugCCCGC-GUUa -5' |
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23481 | 5' | -59.7 | NC_005259.1 | + | 12020 | 0.67 | 0.433333 |
Target: 5'- -gGCCUUGUaC-GGUGCGGGCGgCAAc -3' miRNA: 3'- gaCGGAGCGcGaCCAUGCCCGC-GUUa -5' |
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23481 | 5' | -59.7 | NC_005259.1 | + | 50780 | 0.67 | 0.424049 |
Target: 5'- -gGCCUCGCGCUGGUAguCGGaC-CAu- -3' miRNA: 3'- gaCGGAGCGCGACCAU--GCCcGcGUua -5' |
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23481 | 5' | -59.7 | NC_005259.1 | + | 19190 | 0.68 | 0.413977 |
Target: 5'- gUGcCCUCGacaGCgagcucgUGGUGCGGGCgGCGAc -3' miRNA: 3'- gAC-GGAGCg--CG-------ACCAUGCCCG-CGUUa -5' |
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23481 | 5' | -59.7 | NC_005259.1 | + | 295 | 0.68 | 0.405846 |
Target: 5'- -gGCCUCGCGCUGaucucgACcggGGGUGCGu- -3' miRNA: 3'- gaCGGAGCGCGACca----UG---CCCGCGUua -5' |
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23481 | 5' | -59.7 | NC_005259.1 | + | 34890 | 0.69 | 0.338164 |
Target: 5'- gUGCCUCGgGCaGG-ACGGGcCGCu-- -3' miRNA: 3'- gACGGAGCgCGaCCaUGCCC-GCGuua -5' |
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23481 | 5' | -59.7 | NC_005259.1 | + | 51259 | 0.7 | 0.314987 |
Target: 5'- uUGCggCUCGCGgUGGUcACGGuGCGCGGc -3' miRNA: 3'- gACG--GAGCGCgACCA-UGCC-CGCGUUa -5' |
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23481 | 5' | -59.7 | NC_005259.1 | + | 51661 | 0.7 | 0.307533 |
Target: 5'- uUGCCgcgcgggCGCGUcgGGcACGGGCGCGc- -3' miRNA: 3'- gACGGa------GCGCGa-CCaUGCCCGCGUua -5' |
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23481 | 5' | -59.7 | NC_005259.1 | + | 9397 | 0.7 | 0.285983 |
Target: 5'- -cGCacgCGCGCcccGGUACGGGUGCGc- -3' miRNA: 3'- gaCGga-GCGCGa--CCAUGCCCGCGUua -5' |
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23481 | 5' | -59.7 | NC_005259.1 | + | 29949 | 0.71 | 0.272289 |
Target: 5'- -gGCCcggUCGCgGCUGGUgggGCGGGCGguGa -3' miRNA: 3'- gaCGG---AGCG-CGACCA---UGCCCGCguUa -5' |
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23481 | 5' | -59.7 | NC_005259.1 | + | 52395 | 0.72 | 0.20624 |
Target: 5'- cCUGCCUCGgGCcaucgaGGUGUGGGCGCu-- -3' miRNA: 3'- -GACGGAGCgCGa-----CCAUGCCCGCGuua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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