Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23482 | 5' | -53.1 | NC_005259.1 | + | 59401 | 0.67 | 0.815523 |
Target: 5'- -gCGCGAGCGugaccucgcgcAugGCGUCGGCggccaugaagaacgGCGGUu -3' miRNA: 3'- caGCGCUUGC-----------UugCGUAGCUG--------------UGCCA- -5' |
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23482 | 5' | -53.1 | NC_005259.1 | + | 57064 | 0.66 | 0.899178 |
Target: 5'- -cCGUGAucggguCGAACGCGUCGAgGauguCGGg -3' miRNA: 3'- caGCGCUu-----GCUUGCGUAGCUgU----GCCa -5' |
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23482 | 5' | -53.1 | NC_005259.1 | + | 40064 | 0.66 | 0.89849 |
Target: 5'- gGUCGCG-AUGAcccaugggccgacGCGCAcCGAgCGCGGa -3' miRNA: 3'- -CAGCGCuUGCU-------------UGCGUaGCU-GUGCCa -5' |
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23482 | 5' | -53.1 | NC_005259.1 | + | 66988 | 0.66 | 0.892183 |
Target: 5'- aUCGCGuuguACGccucACGCA-CGACACGcGUg -3' miRNA: 3'- cAGCGCu---UGCu---UGCGUaGCUGUGC-CA- -5' |
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23482 | 5' | -53.1 | NC_005259.1 | + | 61287 | 0.66 | 0.884929 |
Target: 5'- -aUGCcGACGAACGCGagCGGCugauCGGUg -3' miRNA: 3'- caGCGcUUGCUUGCGUa-GCUGu---GCCA- -5' |
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23482 | 5' | -53.1 | NC_005259.1 | + | 66898 | 0.66 | 0.882703 |
Target: 5'- aUCGUGAACGGcgguagauccggcaGCGCGcggugcUCGAC-CGGg -3' miRNA: 3'- cAGCGCUUGCU--------------UGCGU------AGCUGuGCCa -5' |
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23482 | 5' | -53.1 | NC_005259.1 | + | 55012 | 0.66 | 0.877421 |
Target: 5'- cUCGCuuGGCGGuCGUcUCGGCACGGg -3' miRNA: 3'- cAGCGc-UUGCUuGCGuAGCUGUGCCa -5' |
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23482 | 5' | -53.1 | NC_005259.1 | + | 40366 | 0.66 | 0.877421 |
Target: 5'- -cCGCGc-CGAGgGCAugaggucuuccaUCGACACGGg -3' miRNA: 3'- caGCGCuuGCUUgCGU------------AGCUGUGCCa -5' |
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23482 | 5' | -53.1 | NC_005259.1 | + | 6382 | 0.66 | 0.877421 |
Target: 5'- -gCGCGAugGucAACGUcauuuUCGAgGCGGUg -3' miRNA: 3'- caGCGCUugC--UUGCGu----AGCUgUGCCA- -5' |
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23482 | 5' | -53.1 | NC_005259.1 | + | 49994 | 0.67 | 0.844964 |
Target: 5'- aUCGCGuGCGGACGgCugcccUCGuGCACGGg -3' miRNA: 3'- cAGCGCuUGCUUGC-Gu----AGC-UGUGCCa -5' |
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23482 | 5' | -53.1 | NC_005259.1 | + | 56378 | 0.67 | 0.844964 |
Target: 5'- cGUCGCGGGC---CGCGUCGGCugaucgucucgAUGGUg -3' miRNA: 3'- -CAGCGCUUGcuuGCGUAGCUG-----------UGCCA- -5' |
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23482 | 5' | -53.1 | NC_005259.1 | + | 41590 | 0.67 | 0.844964 |
Target: 5'- -gCGUGAGCGGucGCGCGUCGGuguuuCGGa -3' miRNA: 3'- caGCGCUUGCU--UGCGUAGCUgu---GCCa -5' |
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23482 | 5' | -53.1 | NC_005259.1 | + | 24031 | 0.67 | 0.81829 |
Target: 5'- --gGCGAAauggaaccgcCGcAACGuCGUCGGCGCGGUg -3' miRNA: 3'- cagCGCUU----------GC-UUGC-GUAGCUGUGCCA- -5' |
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23482 | 5' | -53.1 | NC_005259.1 | + | 51659 | 0.76 | 0.361542 |
Target: 5'- gGUUGcCGcGCGGGCGCGUCGgGCACGGg -3' miRNA: 3'- -CAGC-GCuUGCUUGCGUAGC-UGUGCCa -5' |
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23482 | 5' | -53.1 | NC_005259.1 | + | 46599 | 0.68 | 0.809001 |
Target: 5'- --gGCGAGC-AGCGCGUCGuugGCGGUc -3' miRNA: 3'- cagCGCUUGcUUGCGUAGCug-UGCCA- -5' |
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23482 | 5' | -53.1 | NC_005259.1 | + | 42591 | 0.68 | 0.780082 |
Target: 5'- -gCGUGAGCGAGCa-GUCGACAuCGGc -3' miRNA: 3'- caGCGCUUGCUUGcgUAGCUGU-GCCa -5' |
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23482 | 5' | -53.1 | NC_005259.1 | + | 36243 | 0.68 | 0.780082 |
Target: 5'- -gCGCGAGCGccUGCGUCGGCAgcgccacgcCGGa -3' miRNA: 3'- caGCGCUUGCuuGCGUAGCUGU---------GCCa -5' |
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23482 | 5' | -53.1 | NC_005259.1 | + | 52820 | 0.68 | 0.770126 |
Target: 5'- --gGCGAGuuuuCGAGgGCAuccUCGACACGGa -3' miRNA: 3'- cagCGCUU----GCUUgCGU---AGCUGUGCCa -5' |
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23482 | 5' | -53.1 | NC_005259.1 | + | 60851 | 0.69 | 0.739463 |
Target: 5'- gGUCGCGAgacucGCGGGuCGCGUUGAaucgaugACGGUg -3' miRNA: 3'- -CAGCGCU-----UGCUU-GCGUAGCUg------UGCCA- -5' |
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23482 | 5' | -53.1 | NC_005259.1 | + | 4345 | 0.69 | 0.718471 |
Target: 5'- cGUCGCGGugAUGAGCGCcggaCGGCACGc- -3' miRNA: 3'- -CAGCGCU--UGCUUGCGua--GCUGUGCca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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