Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23485 | 5' | -60.9 | NC_005259.1 | + | 29945 | 0.66 | 0.49077 |
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Target: 5'- aCGCgg-CCCGgucGCGGCUggugggGCGGGCGg- -3' miRNA: 3'- cGCGacaGGGC---UGCCGA------CGCCCGUau -5' |
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| 23485 | 5' | -60.9 | NC_005259.1 | + | 57342 | 0.66 | 0.480987 |
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Target: 5'- uGCGCUGUCCUcucaagaugagaGAacgGGCaccGUGGGCAg- -3' miRNA: 3'- -CGCGACAGGG------------CUg--CCGa--CGCCCGUau -5' |
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| 23485 | 5' | -60.9 | NC_005259.1 | + | 32548 | 0.66 | 0.432665 |
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Target: 5'- uUGCUGUuggccgcCCCGGCGaGC-GCGGGgAUAg -3' miRNA: 3'- cGCGACA-------GGGCUGC-CGaCGCCCgUAU- -5' |
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| 23485 | 5' | -60.9 | NC_005259.1 | + | 65673 | 0.67 | 0.415397 |
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Target: 5'- uGCGcCUGUCCCuacuCGGCccgacgaGCGGGCcgAg -3' miRNA: 3'- -CGC-GACAGGGcu--GCCGa------CGCCCGuaU- -5' |
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| 23485 | 5' | -60.9 | NC_005259.1 | + | 10335 | 0.67 | 0.389011 |
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Target: 5'- gGCGaCgggCUCGGCGGCaGCGGGCu-- -3' miRNA: 3'- -CGC-GacaGGGCUGCCGaCGCCCGuau -5' |
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| 23485 | 5' | -60.9 | NC_005259.1 | + | 56031 | 0.68 | 0.355585 |
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Target: 5'- cGgGCgggacuUCCUcgGGCGGCUGCGGGUAc- -3' miRNA: 3'- -CgCGac----AGGG--CUGCCGACGCCCGUau -5' |
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| 23485 | 5' | -60.9 | NC_005259.1 | + | 59302 | 0.68 | 0.34755 |
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Target: 5'- aCGCUGUCgUGGCGGCaGCGG-CGa- -3' miRNA: 3'- cGCGACAGgGCUGCCGaCGCCcGUau -5' |
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| 23485 | 5' | -60.9 | NC_005259.1 | + | 42049 | 0.68 | 0.339646 |
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Target: 5'- uGCGCUGUCCU--CGGg-GUGGGCGg- -3' miRNA: 3'- -CGCGACAGGGcuGCCgaCGCCCGUau -5' |
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| 23485 | 5' | -60.9 | NC_005259.1 | + | 51025 | 0.69 | 0.316723 |
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Target: 5'- cGUGCUa--CCGACGaGCgGCGGGCAc- -3' miRNA: 3'- -CGCGAcagGGCUGC-CGaCGCCCGUau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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