miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23486 3' -53.6 NC_005259.1 + 45497 0.66 0.849527
Target:  5'- gUGGCCUUGGUCGcUGUG-GCCa--- -3'
miRNA:   3'- aGCUGGGACUAGCcACACaCGGaaug -5'
23486 3' -53.6 NC_005259.1 + 17303 0.66 0.849527
Target:  5'- cUUGACCCUGuacgccgagCGGUaccccgaggGUGUGCCg--- -3'
miRNA:   3'- -AGCUGGGACua-------GCCA---------CACACGGaaug -5'
23486 3' -53.6 NC_005259.1 + 27476 0.68 0.74428
Target:  5'- -gGGCCggGGUCGGUGUG-GCCa--- -3'
miRNA:   3'- agCUGGgaCUAGCCACACaCGGaaug -5'
23486 3' -53.6 NC_005259.1 + 64783 0.68 0.74428
Target:  5'- gCGGCCCgcugcGAcUGGcUGUGUGCCUcgGCc -3'
miRNA:   3'- aGCUGGGa----CUaGCC-ACACACGGAa-UG- -5'
23486 3' -53.6 NC_005259.1 + 40695 0.69 0.691036
Target:  5'- cCGGCCCgaUGAUCGGg--GUGCCc--- -3'
miRNA:   3'- aGCUGGG--ACUAGCCacaCACGGaaug -5'
23486 3' -53.6 NC_005259.1 + 45450 0.72 0.538466
Target:  5'- cUCGGuCCUUGAUCGGg--GUGCCgaACg -3'
miRNA:   3'- -AGCU-GGGACUAGCCacaCACGGaaUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.