Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23488 | 5' | -60.8 | NC_005259.1 | + | 26839 | 0.71 | 0.239814 |
Target: 5'- gGGCGGUCugCUCGGg---CAGGAUg -3' miRNA: 3'- gCCGCCAGugGAGCCacggGUCCUAg -5' |
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23488 | 5' | -60.8 | NC_005259.1 | + | 27408 | 0.78 | 0.07777 |
Target: 5'- gCGGCGG-CAgCUCGGUGCCCgcgAGGAa- -3' miRNA: 3'- -GCCGCCaGUgGAGCCACGGG---UCCUag -5' |
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23488 | 5' | -60.8 | NC_005259.1 | + | 31946 | 0.79 | 0.06601 |
Target: 5'- aGGCGGUUACCUCgGGUGCCUgAGGuuUCa -3' miRNA: 3'- gCCGCCAGUGGAG-CCACGGG-UCCu-AG- -5' |
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23488 | 5' | -60.8 | NC_005259.1 | + | 68703 | 0.8 | 0.05898 |
Target: 5'- aGGCGGgcagCGCgUCGGUGCCCgagaacuGGGGUCg -3' miRNA: 3'- gCCGCCa---GUGgAGCCACGGG-------UCCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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