Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23489 | 5' | -56.4 | NC_005259.1 | + | 61392 | 0.66 | 0.682298 |
Target: 5'- cGCCCGAuGCCGU-CGguguccucgCGGGCaCGGAc -3' miRNA: 3'- aCGGGCU-CGGCAuGUa--------GCUCGaGUCU- -5' |
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23489 | 5' | -56.4 | NC_005259.1 | + | 63030 | 0.69 | 0.538717 |
Target: 5'- aUGCCCGcgagcugacucgacAGCagcggugCGUACAUCGcgaugAGCUCAGGg -3' miRNA: 3'- -ACGGGC--------------UCG-------GCAUGUAGC-----UCGAGUCU- -5' |
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23489 | 5' | -56.4 | NC_005259.1 | + | 66263 | 0.66 | 0.724593 |
Target: 5'- gGCCCGcGCCGgGCggCG-GCUCAc- -3' miRNA: 3'- aCGGGCuCGGCaUGuaGCuCGAGUcu -5' |
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23489 | 5' | -56.4 | NC_005259.1 | + | 68116 | 0.67 | 0.650057 |
Target: 5'- aGUUCGGGUCGagaGCAgcggCGAGCUCGGc -3' miRNA: 3'- aCGGGCUCGGCa--UGUa---GCUCGAGUCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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