Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23490 | 5' | -54.9 | NC_005259.1 | + | 43066 | 0.69 | 0.62423 |
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Target: 5'- gGGuCGGUGAGGAAcgCGAgcccGUUGcCGCc -3' miRNA: 3'- -CC-GCCGCUCCUUuaGCUa---CAGC-GCGc -5' |
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| 23490 | 5' | -54.9 | NC_005259.1 | + | 46320 | 0.68 | 0.730254 |
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Target: 5'- cGCGGCcccGAGGG--UCGc-GUCGCGCa -3' miRNA: 3'- cCGCCG---CUCCUuuAGCuaCAGCGCGc -5' |
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| 23490 | 5' | -54.9 | NC_005259.1 | + | 46401 | 0.67 | 0.790048 |
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Target: 5'- cGCGGUGAGGgcAUCGAaacCGgGCu -3' miRNA: 3'- cCGCCGCUCCuuUAGCUacaGCgCGc -5' |
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| 23490 | 5' | -54.9 | NC_005259.1 | + | 57443 | 0.68 | 0.719899 |
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Target: 5'- cGCGGCGAGG---UUGGUGcgaCGCuGCGg -3' miRNA: 3'- cCGCCGCUCCuuuAGCUACa--GCG-CGC- -5' |
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| 23490 | 5' | -54.9 | NC_005259.1 | + | 59087 | 0.66 | 0.798586 |
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Target: 5'- uGUGGCGGGcagugauGAcGUUGAUGUcagcaCGCGCGg -3' miRNA: 3'- cCGCCGCUC-------CUuUAGCUACA-----GCGCGC- -5' |
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| 23490 | 5' | -54.9 | NC_005259.1 | + | 60621 | 0.72 | 0.498282 |
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Target: 5'- aGGCGGCGAGGu-GUgGAacUG-CGCGUa -3' miRNA: 3'- -CCGCCGCUCCuuUAgCU--ACaGCGCGc -5' |
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| 23490 | 5' | -54.9 | NC_005259.1 | + | 61033 | 0.7 | 0.592115 |
|
Target: 5'- uGGCGGCGAGG---UCGAccGaCGgGCGg -3' miRNA: 3'- -CCGCCGCUCCuuuAGCUa-CaGCgCGC- -5' |
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| 23490 | 5' | -54.9 | NC_005259.1 | + | 64948 | 0.66 | 0.817976 |
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Target: 5'- gGGUGGCcGGGuuGUCGAUgGUCaUGCa -3' miRNA: 3'- -CCGCCGcUCCuuUAGCUA-CAGcGCGc -5' |
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| 23490 | 5' | -54.9 | NC_005259.1 | + | 67238 | 0.67 | 0.774561 |
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Target: 5'- cGGCGGUGuAGGGGuugagCGGggccucgggcagccgUGUCGCGUc -3' miRNA: 3'- -CCGCCGC-UCCUUua---GCU---------------ACAGCGCGc -5' |
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| 23490 | 5' | -54.9 | NC_005259.1 | + | 67469 | 0.67 | 0.740514 |
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Target: 5'- cGGCgauGGCGGGGAuAUUGAUGacUCGC-Ca -3' miRNA: 3'- -CCG---CCGCUCCUuUAGCUAC--AGCGcGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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