Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23491 | 5' | -56.7 | NC_005259.1 | + | 67104 | 0.66 | 0.673466 |
Target: 5'- uUGGUCCGAGGUgUACU-----GCCCg -3' miRNA: 3'- uACCAGGUUCCAgGUGAgcuacCGGG- -5' |
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23491 | 5' | -56.7 | NC_005259.1 | + | 57034 | 0.67 | 0.598064 |
Target: 5'- -cGGU--GGGGUCgCACUCGucguaGGCCCa -3' miRNA: 3'- uaCCAggUUCCAG-GUGAGCua---CCGGG- -5' |
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23491 | 5' | -56.7 | NC_005259.1 | + | 55035 | 0.66 | 0.684166 |
Target: 5'- -cGGgcugCCGAGGUgCuugcCUCGGUGGCa- -3' miRNA: 3'- uaCCa---GGUUCCAgGu---GAGCUACCGgg -5' |
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23491 | 5' | -56.7 | NC_005259.1 | + | 53732 | 0.72 | 0.3537 |
Target: 5'- gAUGGUgaugucgCCGGGuGUCCACUCGGUGccGCCg -3' miRNA: 3'- -UACCA-------GGUUC-CAGGUGAGCUAC--CGGg -5' |
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23491 | 5' | -56.7 | NC_005259.1 | + | 42396 | 0.68 | 0.555387 |
Target: 5'- -cGGUCCAuGaGUCgucgUACUCGAUGGCg- -3' miRNA: 3'- uaCCAGGUuC-CAG----GUGAGCUACCGgg -5' |
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23491 | 5' | -56.7 | NC_005259.1 | + | 41393 | 0.67 | 0.651964 |
Target: 5'- -cGGUacCCAuGGcgcgCCGCUCGggGuGCCCa -3' miRNA: 3'- uaCCA--GGUuCCa---GGUGAGCuaC-CGGG- -5' |
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23491 | 5' | -56.7 | NC_005259.1 | + | 32441 | 0.7 | 0.425101 |
Target: 5'- -cGGUCuCAGGGUCCAUcaUCu-UGGCCa -3' miRNA: 3'- uaCCAG-GUUCCAGGUG--AGcuACCGGg -5' |
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23491 | 5' | -56.7 | NC_005259.1 | + | 23210 | 0.67 | 0.598064 |
Target: 5'- uGUGGcCCAAGGacggguaccgUCCGCUCGAc-GCCa -3' miRNA: 3'- -UACCaGGUUCC----------AGGUGAGCUacCGGg -5' |
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23491 | 5' | -56.7 | NC_005259.1 | + | 20684 | 0.66 | 0.694818 |
Target: 5'- uGUGGUCgCAcGGcacgCGCUCGGUGGUCg -3' miRNA: 3'- -UACCAG-GUuCCag--GUGAGCUACCGGg -5' |
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23491 | 5' | -56.7 | NC_005259.1 | + | 16155 | 0.68 | 0.534371 |
Target: 5'- -aGGUUguGGuGUCCACUCccGUGGCCg -3' miRNA: 3'- uaCCAGguUC-CAGGUGAGc-UACCGGg -5' |
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23491 | 5' | -56.7 | NC_005259.1 | + | 15139 | 0.7 | 0.463387 |
Target: 5'- -aGGUguaCGGGG-CCAcCUCGGUGGCCg -3' miRNA: 3'- uaCCAg--GUUCCaGGU-GAGCUACCGGg -5' |
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23491 | 5' | -56.7 | NC_005259.1 | + | 14483 | 0.7 | 0.473239 |
Target: 5'- cGUGGUCCGAuccgCUGCUCGGUG-CCCg -3' miRNA: 3'- -UACCAGGUUcca-GGUGAGCUACcGGG- -5' |
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23491 | 5' | -56.7 | NC_005259.1 | + | 7929 | 0.68 | 0.591622 |
Target: 5'- uUGGUCUcguGGGccuUCUugcgcgcccacggcuGCUUGGUGGCCCa -3' miRNA: 3'- uACCAGGu--UCC---AGG---------------UGAGCUACCGGG- -5' |
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23491 | 5' | -56.7 | NC_005259.1 | + | 4467 | 0.67 | 0.619602 |
Target: 5'- uAUGGgCCAAGG-CCAUcggCGGUGGaCCa -3' miRNA: 3'- -UACCaGGUUCCaGGUGa--GCUACCgGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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