Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23492 | 5' | -57.3 | NC_005259.1 | + | 7248 | 0.67 | 0.581463 |
Target: 5'- -gCGCCGGUGucgagUGCGCCaagaaACUGCUc -3' miRNA: 3'- gaGCGGCCACuaca-GCGUGG-----UGACGA- -5' |
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23492 | 5' | -57.3 | NC_005259.1 | + | 15999 | 0.66 | 0.677761 |
Target: 5'- -cCGCCGGUGGc--CGCAUC-CUGCc -3' miRNA: 3'- gaGCGGCCACUacaGCGUGGuGACGa -5' |
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23492 | 5' | -57.3 | NC_005259.1 | + | 45012 | 0.66 | 0.677761 |
Target: 5'- -cCGCCGGg---GcCGCGCCGCccUGCUu -3' miRNA: 3'- gaGCGGCCacuaCaGCGUGGUG--ACGA- -5' |
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23492 | 5' | -57.3 | NC_005259.1 | + | 41657 | 0.66 | 0.677761 |
Target: 5'- -gCGCuCGGUGAgcgGgaucUGCACCGCgUGCg -3' miRNA: 3'- gaGCG-GCCACUa--Ca---GCGUGGUG-ACGa -5' |
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23492 | 5' | -57.3 | NC_005259.1 | + | 47789 | 0.66 | 0.671367 |
Target: 5'- aUCGCgcagcgguccgaCGGUGA-GUCGCgagaccucgcgggccACCGCUGCc -3' miRNA: 3'- gAGCG------------GCCACUaCAGCG---------------UGGUGACGa -5' |
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23492 | 5' | -57.3 | NC_005259.1 | + | 27584 | 0.66 | 0.63496 |
Target: 5'- -aUGCCGuacuUGUCGCACCACcaUGCg -3' miRNA: 3'- gaGCGGCcacuACAGCGUGGUG--ACGa -5' |
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23492 | 5' | -57.3 | NC_005259.1 | + | 56025 | 0.66 | 0.63496 |
Target: 5'- cCUCGCCGGgcggGAcuuccUCGgGCgGCUGCg -3' miRNA: 3'- -GAGCGGCCa---CUac---AGCgUGgUGACGa -5' |
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23492 | 5' | -57.3 | NC_005259.1 | + | 55726 | 0.67 | 0.617795 |
Target: 5'- cCUCGCCcuuguuggcaagcuuGGUGGUGUCGagACCG-UGCg -3' miRNA: 3'- -GAGCGG---------------CCACUACAGCg-UGGUgACGa -5' |
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23492 | 5' | -57.3 | NC_005259.1 | + | 32136 | 0.67 | 0.613507 |
Target: 5'- cCUUGgCGGUGAUGUCGgGCUuauAgaGCUu -3' miRNA: 3'- -GAGCgGCCACUACAGCgUGG---UgaCGA- -5' |
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23492 | 5' | -57.3 | NC_005259.1 | + | 27947 | 0.67 | 0.601729 |
Target: 5'- gCUCGCCGGUGAacggaucUGUCaGCgugGCCAaUGUUc -3' miRNA: 3'- -GAGCGGCCACU-------ACAG-CG---UGGUgACGA- -5' |
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23492 | 5' | -57.3 | NC_005259.1 | + | 18748 | 0.67 | 0.581463 |
Target: 5'- gUCGUCGGUGGUGcUCGaCAUgGcCUGCc -3' miRNA: 3'- gAGCGGCCACUAC-AGC-GUGgU-GACGa -5' |
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23492 | 5' | -57.3 | NC_005259.1 | + | 3188 | 0.67 | 0.581463 |
Target: 5'- gUCGUCGGUGcg--CGCACCACgaUGUg -3' miRNA: 3'- gAGCGGCCACuacaGCGUGGUG--ACGa -5' |
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23492 | 5' | -57.3 | NC_005259.1 | + | 48547 | 0.71 | 0.359369 |
Target: 5'- gUCGCCGGUcuUGUUGUACgcgCGCUGCg -3' miRNA: 3'- gAGCGGCCAcuACAGCGUG---GUGACGa -5' |
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23492 | 5' | -57.3 | NC_005259.1 | + | 56552 | 0.67 | 0.570851 |
Target: 5'- gCUCGUCGGUcucggguucGGUGaUCGuCGCgGCUGCg -3' miRNA: 3'- -GAGCGGCCA---------CUAC-AGC-GUGgUGACGa -5' |
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23492 | 5' | -57.3 | NC_005259.1 | + | 45491 | 0.67 | 0.570851 |
Target: 5'- -cCGCUGGUGGccuugGUCGCugugGCCACcGCg -3' miRNA: 3'- gaGCGGCCACUa----CAGCG----UGGUGaCGa -5' |
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23492 | 5' | -57.3 | NC_005259.1 | + | 40046 | 0.68 | 0.538288 |
Target: 5'- gUCGCCGGUGAUcgggucgGUCGCGaugacCCAUggGCc -3' miRNA: 3'- gAGCGGCCACUA-------CAGCGU-----GGUGa-CGa -5' |
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23492 | 5' | -57.3 | NC_005259.1 | + | 58723 | 0.68 | 0.52895 |
Target: 5'- aUCGCCGGgGGUGUCGUuggCACcgGCa -3' miRNA: 3'- gAGCGGCCaCUACAGCGug-GUGa-CGa -5' |
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23492 | 5' | -57.3 | NC_005259.1 | + | 60505 | 0.68 | 0.508421 |
Target: 5'- -gCGCCGGUGuUGUCGUagugcGCCAUgGCc -3' miRNA: 3'- gaGCGGCCACuACAGCG-----UGGUGaCGa -5' |
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23492 | 5' | -57.3 | NC_005259.1 | + | 61192 | 0.69 | 0.498282 |
Target: 5'- cCUCGCCGGUc-UGcUUGCACCACaucGCa -3' miRNA: 3'- -GAGCGGCCAcuAC-AGCGUGGUGa--CGa -5' |
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23492 | 5' | -57.3 | NC_005259.1 | + | 30770 | 0.7 | 0.439531 |
Target: 5'- -aCGCCGGUG-----GCACCGCUGCc -3' miRNA: 3'- gaGCGGCCACuacagCGUGGUGACGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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