Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23492 | 5' | -57.3 | NC_005259.1 | + | 60505 | 0.68 | 0.508421 |
Target: 5'- -gCGCCGGUGuUGUCGUagugcGCCAUgGCc -3' miRNA: 3'- gaGCGGCCACuACAGCG-----UGGUGaCGa -5' |
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23492 | 5' | -57.3 | NC_005259.1 | + | 60931 | 0.71 | 0.384933 |
Target: 5'- gCUCgGCCuuGGUGAUcGUCuGCACCACaUGCg -3' miRNA: 3'- -GAG-CGG--CCACUA-CAG-CGUGGUG-ACGa -5' |
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23492 | 5' | -57.3 | NC_005259.1 | + | 61192 | 0.69 | 0.498282 |
Target: 5'- cCUCGCCGGUc-UGcUUGCACCACaucGCa -3' miRNA: 3'- -GAGCGGCCAcuAC-AGCGUGGUGa--CGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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