Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23495 | 3' | -55.6 | NC_005259.1 | + | 67415 | 0.66 | 0.708873 |
Target: 5'- -cCGacACCAUCGGGGUGCCgGgCUCa -3' miRNA: 3'- aaGCacUGGUGGUUCCACGGgUaGAG- -5' |
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23495 | 3' | -55.6 | NC_005259.1 | + | 64339 | 0.68 | 0.586364 |
Target: 5'- cUCG-GACUGCCAcucguugcgggcGGUGCCCAUCa- -3' miRNA: 3'- aAGCaCUGGUGGUu-----------CCACGGGUAGag -5' |
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23495 | 3' | -55.6 | NC_005259.1 | + | 62116 | 0.66 | 0.730195 |
Target: 5'- cUCGUaGucagUCACCGAGGUGaUCGUCUCg -3' miRNA: 3'- aAGCA-Cu---GGUGGUUCCACgGGUAGAG- -5' |
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23495 | 3' | -55.6 | NC_005259.1 | + | 60384 | 0.66 | 0.68725 |
Target: 5'- aUCGccgGACagCGgCGAGGcgagGCCCAUCUCg -3' miRNA: 3'- aAGCa--CUG--GUgGUUCCa---CGGGUAGAG- -5' |
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23495 | 3' | -55.6 | NC_005259.1 | + | 58665 | 0.66 | 0.698094 |
Target: 5'- -cCG-GugCACCGGGGUGCagcuuCCAUCg- -3' miRNA: 3'- aaGCaCugGUGGUUCCACG-----GGUAGag -5' |
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23495 | 3' | -55.6 | NC_005259.1 | + | 57691 | 0.66 | 0.698094 |
Target: 5'- -cCGUGGCCagguuGCCGGGcuUGCCCGaCUCg -3' miRNA: 3'- aaGCACUGG-----UGGUUCc-ACGGGUaGAG- -5' |
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23495 | 3' | -55.6 | NC_005259.1 | + | 56905 | 0.7 | 0.462205 |
Target: 5'- -gCGUGGCCGCCGcagcaGGcGUGCCCGcgUCg -3' miRNA: 3'- aaGCACUGGUGGU-----UC-CACGGGUagAG- -5' |
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23495 | 3' | -55.6 | NC_005259.1 | + | 55028 | 0.71 | 0.442485 |
Target: 5'- cUCGgcacgGGCUGCCGAGGUGCUUGcCUCg -3' miRNA: 3'- aAGCa----CUGGUGGUUCCACGGGUaGAG- -5' |
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23495 | 3' | -55.6 | NC_005259.1 | + | 53520 | 0.72 | 0.386328 |
Target: 5'- cUCGUGGCCAgCGGcacgggcucGGUGCCCGgugucggCUCg -3' miRNA: 3'- aAGCACUGGUgGUU---------CCACGGGUa------GAG- -5' |
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23495 | 3' | -55.6 | NC_005259.1 | + | 52400 | 0.66 | 0.719577 |
Target: 5'- cUCG-GGCCAUCGAGGUgugggcGCUCGaCUCg -3' miRNA: 3'- aAGCaCUGGUGGUUCCA------CGGGUaGAG- -5' |
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23495 | 3' | -55.6 | NC_005259.1 | + | 49541 | 0.66 | 0.740715 |
Target: 5'- -aCGUGGCCACCucaGUGCCgGgcugUUCg -3' miRNA: 3'- aaGCACUGGUGGuucCACGGgUa---GAG- -5' |
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23495 | 3' | -55.6 | NC_005259.1 | + | 36132 | 0.66 | 0.698094 |
Target: 5'- gUUG-GGCCGCCGGGcUGUCCGUCg- -3' miRNA: 3'- aAGCaCUGGUGGUUCcACGGGUAGag -5' |
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23495 | 3' | -55.6 | NC_005259.1 | + | 35227 | 0.67 | 0.665416 |
Target: 5'- -aCGUGACCGCCAcGcGUGCa-GUCUg -3' miRNA: 3'- aaGCACUGGUGGUuC-CACGggUAGAg -5' |
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23495 | 3' | -55.6 | NC_005259.1 | + | 23153 | 0.66 | 0.730195 |
Target: 5'- -gCGaGACCACCcucGGUGUCCcgUUCa -3' miRNA: 3'- aaGCaCUGGUGGuu-CCACGGGuaGAG- -5' |
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23495 | 3' | -55.6 | NC_005259.1 | + | 21963 | 0.69 | 0.52393 |
Target: 5'- -gCGUGAcCCGCguGGGUGUCguUCUCc -3' miRNA: 3'- aaGCACU-GGUGguUCCACGGguAGAG- -5' |
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23495 | 3' | -55.6 | NC_005259.1 | + | 16415 | 0.76 | 0.223452 |
Target: 5'- ----aGcCCACCGAGGUGCCCGUCg- -3' miRNA: 3'- aagcaCuGGUGGUUCCACGGGUAGag -5' |
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23495 | 3' | -55.6 | NC_005259.1 | + | 16173 | 0.68 | 0.599493 |
Target: 5'- -cCGUGGCCGCCGAGGUccaagagaucauGUCCAc--- -3' miRNA: 3'- aaGCACUGGUGGUUCCA------------CGGGUagag -5' |
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23495 | 3' | -55.6 | NC_005259.1 | + | 15422 | 0.68 | 0.577641 |
Target: 5'- -gCGUG-CCACCAAG--GCCgAUCUCg -3' miRNA: 3'- aaGCACuGGUGGUUCcaCGGgUAGAG- -5' |
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23495 | 3' | -55.6 | NC_005259.1 | + | 11450 | 0.66 | 0.698094 |
Target: 5'- cUCGcuGCCGCC--GGUGCCUaucGUCUCg -3' miRNA: 3'- aAGCacUGGUGGuuCCACGGG---UAGAG- -5' |
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23495 | 3' | -55.6 | NC_005259.1 | + | 5704 | 0.67 | 0.676354 |
Target: 5'- cUCGUG-CCGCCAAGGccaGCgCCGgacggcggccauUCUCg -3' miRNA: 3'- aAGCACuGGUGGUUCCa--CG-GGU------------AGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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