Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23495 | 5' | -57.9 | NC_005259.1 | + | 60928 | 0.76 | 0.191959 |
Target: 5'- -uGAGCUCGGCCUUGGUGAucGUcugcACCa- -3' miRNA: 3'- guCUCGAGCCGGAGCCACU--CA----UGGcc -5' |
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23495 | 5' | -57.9 | NC_005259.1 | + | 61926 | 0.74 | 0.241202 |
Target: 5'- uCGGcAGCUCGGUcuCUCGcGcGGGUGCCGGg -3' miRNA: 3'- -GUC-UCGAGCCG--GAGC-CaCUCAUGGCC- -5' |
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23495 | 5' | -57.9 | NC_005259.1 | + | 58233 | 0.74 | 0.259817 |
Target: 5'- -cGAGCUCGGgCUCGGUGaAGaACCa- -3' miRNA: 3'- guCUCGAGCCgGAGCCAC-UCaUGGcc -5' |
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23495 | 5' | -57.9 | NC_005259.1 | + | 67390 | 0.71 | 0.370538 |
Target: 5'- ---cGCUUGGCCUCGG-GGGUGuCaCGGg -3' miRNA: 3'- gucuCGAGCCGGAGCCaCUCAU-G-GCC- -5' |
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23495 | 5' | -57.9 | NC_005259.1 | + | 27042 | 0.71 | 0.387533 |
Target: 5'- -uGAGCUUGGCCUUGGccUGAGcgaCGGu -3' miRNA: 3'- guCUCGAGCCGGAGCC--ACUCaugGCC- -5' |
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23495 | 5' | -57.9 | NC_005259.1 | + | 36916 | 0.7 | 0.441482 |
Target: 5'- aCGGGGauaUCGuGCCgaCGGUGAGUguugcugucACCGGg -3' miRNA: 3'- -GUCUCg--AGC-CGGa-GCCACUCA---------UGGCC- -5' |
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23495 | 5' | -57.9 | NC_005259.1 | + | 8789 | 0.69 | 0.46039 |
Target: 5'- -uGAGCUCGGCCcgcUUGGcGAGgaucuucucgcGCCGGg -3' miRNA: 3'- guCUCGAGCCGG---AGCCaCUCa----------UGGCC- -5' |
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23495 | 5' | -57.9 | NC_005259.1 | + | 59132 | 0.68 | 0.550174 |
Target: 5'- gCAGAGCgUCGaG-CUCGGcagcGGUGCCGGg -3' miRNA: 3'- -GUCUCG-AGC-CgGAGCCac--UCAUGGCC- -5' |
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23495 | 5' | -57.9 | NC_005259.1 | + | 13406 | 0.67 | 0.581379 |
Target: 5'- ---cGCUCGGCCUuccUGGccgGGGUcgccGCCGGg -3' miRNA: 3'- gucuCGAGCCGGA---GCCa--CUCA----UGGCC- -5' |
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23495 | 5' | -57.9 | NC_005259.1 | + | 25507 | 0.67 | 0.601334 |
Target: 5'- gAGGGCaaaaaGGCCcucgaccguggcaUCGcGUGGGUGCUGGg -3' miRNA: 3'- gUCUCGag---CCGG-------------AGC-CACUCAUGGCC- -5' |
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23495 | 5' | -57.9 | NC_005259.1 | + | 26797 | 0.66 | 0.655174 |
Target: 5'- cCAGcGCcgccgcccggUUGGCCgguaccggCGGUG-GUGCCGGg -3' miRNA: 3'- -GUCuCG----------AGCCGGa-------GCCACuCAUGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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