Results 21 - 40 of 41 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23498 | 3' | -55.2 | NC_005259.1 | + | 13421 | 0.68 | 0.675379 |
Target: 5'- gCCGggGUcgccgccGggGCCgCGCuAGGCGugaccgucggcGCGGCg -3' miRNA: 3'- -GGCuuCA-------CaaUGG-GCG-UCCGC-----------UGCCG- -5' |
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23498 | 3' | -55.2 | NC_005259.1 | + | 20977 | 0.68 | 0.685973 |
Target: 5'- gCCGggGUGacaacUGCgUGUGGGCcuacucgGGCGGCg -3' miRNA: 3'- -GGCuuCACa----AUGgGCGUCCG-------CUGCCG- -5' |
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23498 | 3' | -55.2 | NC_005259.1 | + | 8652 | 0.68 | 0.68703 |
Target: 5'- aCGAGGcUGUggucgggcUGCCaCGCGGGCaACGGg -3' miRNA: 3'- gGCUUC-ACA--------AUGG-GCGUCCGcUGCCg -5' |
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23498 | 3' | -55.2 | NC_005259.1 | + | 8976 | 0.68 | 0.697571 |
Target: 5'- gCCGGGacGUaggUGCCaGC-GGCGACGGCa -3' miRNA: 3'- -GGCUU--CAca-AUGGgCGuCCGCUGCCG- -5' |
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23498 | 3' | -55.2 | NC_005259.1 | + | 48035 | 0.68 | 0.708052 |
Target: 5'- aCGA--UGUUGaccacCCCGguGGCcACGGCg -3' miRNA: 3'- gGCUucACAAU-----GGGCguCCGcUGCCG- -5' |
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23498 | 3' | -55.2 | NC_005259.1 | + | 27377 | 0.68 | 0.718463 |
Target: 5'- cCCGGcacGGUG--ACCgGCGGcggugucggcaGCGGCGGCa -3' miRNA: 3'- -GGCU---UCACaaUGGgCGUC-----------CGCUGCCG- -5' |
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23498 | 3' | -55.2 | NC_005259.1 | + | 37019 | 0.68 | 0.718463 |
Target: 5'- gCCGggGaacaugacaaUGUcgaucuuggUGCCCGCcaccgcccacGGCGGCGGUg -3' miRNA: 3'- -GGCuuC----------ACA---------AUGGGCGu---------CCGCUGCCG- -5' |
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23498 | 3' | -55.2 | NC_005259.1 | + | 26382 | 0.67 | 0.728793 |
Target: 5'- gCGAGccUGUUGCUCGCgauuccgacgccGGGCG-CGGCg -3' miRNA: 3'- gGCUUc-ACAAUGGGCG------------UCCGCuGCCG- -5' |
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23498 | 3' | -55.2 | NC_005259.1 | + | 20523 | 0.67 | 0.728793 |
Target: 5'- cCCGAgcacgGGUGcgcUGCCCGCAGcGUGGUGGa -3' miRNA: 3'- -GGCU-----UCACa--AUGGGCGUC-CGCUGCCg -5' |
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23498 | 3' | -55.2 | NC_005259.1 | + | 34540 | 0.67 | 0.736992 |
Target: 5'- aCCGAGGcagccucagcUugCCGUcgGGGcCGACGGCg -3' miRNA: 3'- -GGCUUCaca-------AugGGCG--UCC-GCUGCCG- -5' |
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23498 | 3' | -55.2 | NC_005259.1 | + | 8754 | 0.67 | 0.769096 |
Target: 5'- gUCGAGGUcGagGCCCuuggcCAGcGCGGCGGUg -3' miRNA: 3'- -GGCUUCA-CaaUGGGc----GUC-CGCUGCCG- -5' |
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23498 | 3' | -55.2 | NC_005259.1 | + | 45518 | 0.66 | 0.788491 |
Target: 5'- aCCGcgccGGUGUUGgCCGCcguGGCuGCGaGCg -3' miRNA: 3'- -GGCu---UCACAAUgGGCGu--CCGcUGC-CG- -5' |
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23498 | 3' | -55.2 | NC_005259.1 | + | 58726 | 0.66 | 0.797963 |
Target: 5'- gCCGggGgugucGUUggcACCgGCAccGGCGAaguuCGGCg -3' miRNA: 3'- -GGCuuCa----CAA---UGGgCGU--CCGCU----GCCG- -5' |
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23498 | 3' | -55.2 | NC_005259.1 | + | 60847 | 0.66 | 0.804497 |
Target: 5'- gCGAGGUcgcgagACUCGCGGGUcgcguugaaucgauGACGGUg -3' miRNA: 3'- gGCUUCAcaa---UGGGCGUCCG--------------CUGCCG- -5' |
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23498 | 3' | -55.2 | NC_005259.1 | + | 24561 | 0.66 | 0.807271 |
Target: 5'- gCGggGUG-UACUCGUGGGC-ACcGCa -3' miRNA: 3'- gGCuuCACaAUGGGCGUCCGcUGcCG- -5' |
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23498 | 3' | -55.2 | NC_005259.1 | + | 31673 | 0.66 | 0.807271 |
Target: 5'- cUCGAcgGG-GUUGCCCG-AGGCauccucGGCGGUg -3' miRNA: 3'- -GGCU--UCaCAAUGGGCgUCCG------CUGCCG- -5' |
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23498 | 3' | -55.2 | NC_005259.1 | + | 29377 | 0.66 | 0.815501 |
Target: 5'- gUCGAGGUc-UACCCGCcgaccuacaccgaGGGCcuCGGCg -3' miRNA: 3'- -GGCUUCAcaAUGGGCG-------------UCCGcuGCCG- -5' |
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23498 | 3' | -55.2 | NC_005259.1 | + | 182 | 0.66 | 0.816406 |
Target: 5'- aCCGAcucGGUcaucuaugGCgCGUGGcGCGACGGCa -3' miRNA: 3'- -GGCU---UCAcaa-----UGgGCGUC-CGCUGCCG- -5' |
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23498 | 3' | -55.2 | NC_005259.1 | + | 61394 | 0.66 | 0.824472 |
Target: 5'- cCCGAugccgucGGUGUc-CUCGCGGGC-ACGGa -3' miRNA: 3'- -GGCU-------UCACAauGGGCGUCCGcUGCCg -5' |
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23498 | 3' | -55.2 | NC_005259.1 | + | 65757 | 0.66 | 0.825359 |
Target: 5'- gCCGAguAGUGgUACCCguacagaucGCAGuGCGccAUGGCc -3' miRNA: 3'- -GGCU--UCACaAUGGG---------CGUC-CGC--UGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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