Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2350 | 3' | -59.2 | NC_001416.1 | + | 10338 | 0.69 | 0.283654 |
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Target: 5'- -gAGCACGGU-GUACGUCaGCCCGGa- -3' miRNA: 3'- ugUCGUGUCGuCGUGUAG-CGGGCCga -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 16998 | 0.69 | 0.276565 |
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Target: 5'- cCGGCAUAgucaucaucGCAGauuucaauaACAUCGCCCGGUa -3' miRNA: 3'- uGUCGUGU---------CGUCg--------UGUAGCGGGCCGa -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 14765 | 0.7 | 0.243233 |
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Target: 5'- uAUGGUGCAGCAGCucgcCGUCGCCgcaguaaauugCGGCg -3' miRNA: 3'- -UGUCGUGUCGUCGu---GUAGCGG-----------GCCGa -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 16586 | 0.7 | 0.243233 |
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Target: 5'- cCGGCAUA-CAGCGCAUCGCCa-GCa -3' miRNA: 3'- uGUCGUGUcGUCGUGUAGCGGgcCGa -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 14233 | 0.7 | 0.219031 |
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Target: 5'- aGCcGCACgacuccgcuGGC-GCACAUCGCCgGGCg -3' miRNA: 3'- -UGuCGUG---------UCGuCGUGUAGCGGgCCGa -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 14127 | 0.72 | 0.167408 |
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Target: 5'- gACAGaauCAGCgauucuGGCGCA-CGCCCGGCg -3' miRNA: 3'- -UGUCgu-GUCG------UCGUGUaGCGGGCCGa -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 4762 | 0.74 | 0.130559 |
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Target: 5'- uACGGCu--GCAGCGCccgCGUCCGGCUg -3' miRNA: 3'- -UGUCGuguCGUCGUGua-GCGGGCCGA- -5' |
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| 2350 | 3' | -59.2 | NC_001416.1 | + | 14702 | 1.07 | 0.00036 |
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Target: 5'- aACAGCACAGCAGCACAUCGCCCGGCUg -3' miRNA: 3'- -UGUCGUGUCGUCGUGUAGCGGGCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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