Results 21 - 40 of 42 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23500 | 5' | -53.5 | NC_005259.1 | + | 22075 | 0.69 | 0.72964 |
Target: 5'- cCGUGGUGAgcUCAUCGACcagaugGCGGg -3' miRNA: 3'- cGCGCCACU--AGUGGCUGuagua-CGCC- -5' |
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23500 | 5' | -53.5 | NC_005259.1 | + | 20967 | 0.69 | 0.72964 |
Target: 5'- aCGCGGU-AUCGCCGGgGUgacaacugCGUGUGGg -3' miRNA: 3'- cGCGCCAcUAGUGGCUgUA--------GUACGCC- -5' |
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23500 | 5' | -53.5 | NC_005259.1 | + | 58477 | 0.7 | 0.68632 |
Target: 5'- uGCGUGGcuGUCGCCGACGaCAUcgaccucGCGGu -3' miRNA: 3'- -CGCGCCacUAGUGGCUGUaGUA-------CGCC- -5' |
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23500 | 5' | -53.5 | NC_005259.1 | + | 18335 | 0.7 | 0.676672 |
Target: 5'- gGUGCGuucGUGGUCgacGCCGACGgcgaCAUGUGGc -3' miRNA: 3'- -CGCGC---CACUAG---UGGCUGUa---GUACGCC- -5' |
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23500 | 5' | -53.5 | NC_005259.1 | + | 35670 | 0.7 | 0.676672 |
Target: 5'- aGCGCGGUGGUggugcaCAgCGACGcCGUGCc- -3' miRNA: 3'- -CGCGCCACUA------GUgGCUGUaGUACGcc -5' |
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23500 | 5' | -53.5 | NC_005259.1 | + | 39835 | 0.7 | 0.655126 |
Target: 5'- aGCGCGuUGGUCGgCGACAggAUGCcGGu -3' miRNA: 3'- -CGCGCcACUAGUgGCUGUagUACG-CC- -5' |
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23500 | 5' | -53.5 | NC_005259.1 | + | 51266 | 0.71 | 0.644317 |
Target: 5'- uCGCGGUGGUCACgGugcGCggCAaGCGGc -3' miRNA: 3'- cGCGCCACUAGUGgC---UGuaGUaCGCC- -5' |
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23500 | 5' | -53.5 | NC_005259.1 | + | 27105 | 0.71 | 0.644317 |
Target: 5'- aUGCGGUGG--GCUG-UGUCAUGCGGa -3' miRNA: 3'- cGCGCCACUagUGGCuGUAGUACGCC- -5' |
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23500 | 5' | -53.5 | NC_005259.1 | + | 6065 | 0.71 | 0.644317 |
Target: 5'- uGCGCGGUGAUCGCCGccgaACAggacGCc- -3' miRNA: 3'- -CGCGCCACUAGUGGC----UGUaguaCGcc -5' |
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23500 | 5' | -53.5 | NC_005259.1 | + | 17473 | 0.71 | 0.633497 |
Target: 5'- cCGUGGUGA-CGcCCGGC--CAUGCGGg -3' miRNA: 3'- cGCGCCACUaGU-GGCUGuaGUACGCC- -5' |
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23500 | 5' | -53.5 | NC_005259.1 | + | 10732 | 0.71 | 0.608622 |
Target: 5'- aUGuCGGUGAUCACCGcGCGcugccgggcgaucaUCAUGuCGGg -3' miRNA: 3'- cGC-GCCACUAGUGGC-UGU--------------AGUAC-GCC- -5' |
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23500 | 5' | -53.5 | NC_005259.1 | + | 6210 | 0.71 | 0.601067 |
Target: 5'- aGCGgGGUGAgccgccgCGCCGACAcCGUgaccGUGGg -3' miRNA: 3'- -CGCgCCACUa------GUGGCUGUaGUA----CGCC- -5' |
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23500 | 5' | -53.5 | NC_005259.1 | + | 1519 | 0.72 | 0.578489 |
Target: 5'- aCGCuGUcacugacGAUCACCGGCAUCAU-CGGg -3' miRNA: 3'- cGCGcCA-------CUAGUGGCUGUAGUAcGCC- -5' |
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23500 | 5' | -53.5 | NC_005259.1 | + | 66213 | 0.72 | 0.547635 |
Target: 5'- gGCGCGGUcg-CGCCGACgcacacgcgGUCGUGCa- -3' miRNA: 3'- -CGCGCCAcuaGUGGCUG---------UAGUACGcc -5' |
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23500 | 5' | -53.5 | NC_005259.1 | + | 23288 | 0.73 | 0.505993 |
Target: 5'- gGCGCGGUGGUUggguAUCGGgGUCcgGCGcGg -3' miRNA: 3'- -CGCGCCACUAG----UGGCUgUAGuaCGC-C- -5' |
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23500 | 5' | -53.5 | NC_005259.1 | + | 66924 | 0.73 | 0.49579 |
Target: 5'- cGCGCGGUGcUCgACCGGgGUgA-GCGGg -3' miRNA: 3'- -CGCGCCACuAG-UGGCUgUAgUaCGCC- -5' |
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23500 | 5' | -53.5 | NC_005259.1 | + | 62695 | 0.73 | 0.485681 |
Target: 5'- aGUGCGGUGuccCAuCCGGCgAUCAgGCGGg -3' miRNA: 3'- -CGCGCCACua-GU-GGCUG-UAGUaCGCC- -5' |
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23500 | 5' | -53.5 | NC_005259.1 | + | 27061 | 0.74 | 0.465765 |
Target: 5'- aGCGaCGGUcacggcGAcaUCGCCGACAUUcUGCGGc -3' miRNA: 3'- -CGC-GCCA------CU--AGUGGCUGUAGuACGCC- -5' |
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23500 | 5' | -53.5 | NC_005259.1 | + | 11747 | 0.74 | 0.465765 |
Target: 5'- uGCGCagacggucauaGGUGAUCACCGAC-UCGacgGCGa -3' miRNA: 3'- -CGCG-----------CCACUAGUGGCUGuAGUa--CGCc -5' |
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23500 | 5' | -53.5 | NC_005259.1 | + | 14424 | 0.75 | 0.417007 |
Target: 5'- aGgGCGGUGccacagcAUgACCGACAUCAucgacgccccugUGCGGg -3' miRNA: 3'- -CgCGCCAC-------UAgUGGCUGUAGU------------ACGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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