Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23501 | 3' | -55.8 | NC_005259.1 | + | 29966 | 0.74 | 0.285042 |
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Target: 5'- ---uGGGGCgGGCGguGACCGUCCa-- -3' miRNA: 3'- guuuCUCCG-CCGCguCUGGUAGGagu -5' |
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| 23501 | 3' | -55.8 | NC_005259.1 | + | 42378 | 0.69 | 0.515824 |
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Target: 5'- uGAGGaAGGCGG-GCAGGCCgGUCCa-- -3' miRNA: 3'- gUUUC-UCCGCCgCGUCUGG-UAGGagu -5' |
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| 23501 | 3' | -55.8 | NC_005259.1 | + | 1234 | 0.69 | 0.526277 |
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Target: 5'- ---cGAGGaCGGCuGCaAGACCggCCUCGg -3' miRNA: 3'- guuuCUCC-GCCG-CG-UCUGGuaGGAGU- -5' |
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| 23501 | 3' | -55.8 | NC_005259.1 | + | 49370 | 0.68 | 0.612148 |
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Target: 5'- uGAAGAGGcCGGUG-AGAUCGUUCUUg -3' miRNA: 3'- gUUUCUCC-GCCGCgUCUGGUAGGAGu -5' |
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| 23501 | 3' | -55.8 | NC_005259.1 | + | 29906 | 0.67 | 0.633953 |
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Target: 5'- -cAAGGGGUGGCuGCccgAGACCAUCgaCGc -3' miRNA: 3'- guUUCUCCGCCG-CG---UCUGGUAGgaGU- -5' |
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| 23501 | 3' | -55.8 | NC_005259.1 | + | 15157 | 0.67 | 0.688261 |
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Target: 5'- -----cGGUGGCcgagGCcgAGGCCAUCCUCGa -3' miRNA: 3'- guuucuCCGCCG----CG--UCUGGUAGGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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