miRNA: 3'- uuCGCUGUUGUAGCa--GCGcaa-GGGCG- -5'
miRNA: 3'- uUCGCUGU-UGUAGCaGCGCAAGggCG- -5'
miRNA: 3'- uUCGCugUUGUAGCAGCG---CAAGGgCG- -5'
miRNA: 3'- uuCGCUGUUGUAGCAGCGcAAGG-GCG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23503 | 5' | -53.5 | NC_005259.1 | + | 5672 | 0.72 | 0.542394 |
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Target: 5'- -cGCGACAACAUCGcccCGCacugCCUGCu -3' miRNA: 3'- uuCGCUGUUGUAGCa--GCGcaa-GGGCG- -5' |
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| 23503 | 5' | -53.5 | NC_005259.1 | + | 34030 | 0.75 | 0.358286 |
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Target: 5'- cGGCGGCGuGCGUCGgCGCGUUCuuGUu -3' miRNA: 3'- uUCGCUGU-UGUAGCaGCGCAAGggCG- -5' |
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| 23503 | 5' | -53.5 | NC_005259.1 | + | 1952 | 0.79 | 0.228449 |
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Target: 5'- cGGCGugAGCGUUGUCGUgacGUUCCgGCg -3' miRNA: 3'- uUCGCugUUGUAGCAGCG---CAAGGgCG- -5' |
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| 23503 | 5' | -53.5 | NC_005259.1 | + | 9873 | 0.79 | 0.205618 |
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Target: 5'- -cGCGACGAUGUUGUCGCuUUCCuCGCg -3' miRNA: 3'- uuCGCUGUUGUAGCAGCGcAAGG-GCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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