Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23505 | 3' | -60.7 | NC_005259.1 | + | 35737 | 0.69 | 0.351971 |
Target: 5'- ---aCCCGGCGC--UGCCCGgccCCACGu -3' miRNA: 3'- gcaaGGGCCGUGccACGGGCa--GGUGC- -5' |
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23505 | 3' | -60.7 | NC_005259.1 | + | 19171 | 0.66 | 0.542531 |
Target: 5'- gGUUCgUCGGCgcucaACGGUGCCC-UCgACa -3' miRNA: 3'- gCAAG-GGCCG-----UGCCACGGGcAGgUGc -5' |
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23505 | 3' | -60.7 | NC_005259.1 | + | 40687 | 0.66 | 0.502924 |
Target: 5'- gGUgcUCaCCGGCccgaugaucGgGGUGCCCGccaCCACGg -3' miRNA: 3'- gCA--AG-GGCCG---------UgCCACGGGCa--GGUGC- -5' |
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23505 | 3' | -60.7 | NC_005259.1 | + | 3166 | 0.67 | 0.49225 |
Target: 5'- gGUgaugCCCGGCGaugucaaggucguCGGUGCgCGcaCCACGa -3' miRNA: 3'- gCAa---GGGCCGU-------------GCCACGgGCa-GGUGC- -5' |
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23505 | 3' | -60.7 | NC_005259.1 | + | 40561 | 0.67 | 0.483595 |
Target: 5'- gCGcgCCC-GCGCGGUGUugCUGUCgGCGg -3' miRNA: 3'- -GCaaGGGcCGUGCCACG--GGCAGgUGC- -5' |
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23505 | 3' | -60.7 | NC_005259.1 | + | 24051 | 0.67 | 0.483595 |
Target: 5'- aCGUcgUCGGCGCGGUGCUcaaucuCGUgcaCCGCGa -3' miRNA: 3'- -GCAagGGCCGUGCCACGG------GCA---GGUGC- -5' |
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23505 | 3' | -60.7 | NC_005259.1 | + | 24814 | 0.68 | 0.43693 |
Target: 5'- gGUgcCCCGGCACGccGUGCCCGcaCCGa- -3' miRNA: 3'- gCAa-GGGCCGUGC--CACGGGCa-GGUgc -5' |
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23505 | 3' | -60.7 | NC_005259.1 | + | 30201 | 0.68 | 0.419 |
Target: 5'- ---cCCCGu--CGGUGCCCG-CCGCGa -3' miRNA: 3'- gcaaGGGCcguGCCACGGGCaGGUGC- -5' |
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23505 | 3' | -60.7 | NC_005259.1 | + | 22464 | 0.68 | 0.410205 |
Target: 5'- uCGUgcgCCC-GCGCGGUcugaCCGUCCGCa -3' miRNA: 3'- -GCAa--GGGcCGUGCCAcg--GGCAGGUGc -5' |
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23505 | 3' | -60.7 | NC_005259.1 | + | 9130 | 0.75 | 0.14575 |
Target: 5'- ---cCCCGGUACGGgcagccgugcucgUGCCCGUCgACGg -3' miRNA: 3'- gcaaGGGCCGUGCC-------------ACGGGCAGgUGC- -5' |
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23505 | 3' | -60.7 | NC_005259.1 | + | 45630 | 0.69 | 0.34961 |
Target: 5'- cCGggCCgGGCAgcgcgcCGGUGCcgccgugaacaccgCCGUCCGCGu -3' miRNA: 3'- -GCaaGGgCCGU------GCCACG--------------GGCAGGUGC- -5' |
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23505 | 3' | -60.7 | NC_005259.1 | + | 34579 | 0.69 | 0.344145 |
Target: 5'- gGUUCUCGgacaggcagacGCGCGGUGUcgacaucacUCGUCCGCGa -3' miRNA: 3'- gCAAGGGC-----------CGUGCCACG---------GGCAGGUGC- -5' |
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23505 | 3' | -60.7 | NC_005259.1 | + | 20516 | 0.7 | 0.306911 |
Target: 5'- aCGgugUCCCGaGCACgGGUGCgCUGcCCGCa -3' miRNA: 3'- -GCa--AGGGC-CGUG-CCACG-GGCaGGUGc -5' |
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23505 | 3' | -60.7 | NC_005259.1 | + | 37275 | 0.7 | 0.306911 |
Target: 5'- aGgccgCCCGGCaggacuugcaugGCGGUGCCCG-CCGgGu -3' miRNA: 3'- gCaa--GGGCCG------------UGCCACGGGCaGGUgC- -5' |
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23505 | 3' | -60.7 | NC_005259.1 | + | 37060 | 0.71 | 0.28609 |
Target: 5'- ---cCCaCGGCgGCGGUGuCCaCGUCCACGc -3' miRNA: 3'- gcaaGG-GCCG-UGCCAC-GG-GCAGGUGC- -5' |
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23505 | 3' | -60.7 | NC_005259.1 | + | 19963 | 0.73 | 0.203491 |
Target: 5'- aCGgcggCUCGGCGgugugGGUGCCCGUCUACa -3' miRNA: 3'- -GCaa--GGGCCGUg----CCACGGGCAGGUGc -5' |
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23505 | 3' | -60.7 | NC_005259.1 | + | 27373 | 0.74 | 0.166206 |
Target: 5'- gGUgCCCGGCACGGUGaCCGgCgGCGg -3' miRNA: 3'- gCAaGGGCCGUGCCACgGGCaGgUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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