miRNA display CGI


Results 1 - 11 of 11 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23505 5' -53.2 NC_005259.1 + 51276 0.66 0.863985
Target:  5'- cACgGUGcgCGGCAAGCGGcagcGCCCGa-- -3'
miRNA:   3'- -UGgCACuaGCUGUUUGCU----CGGGUcuu -5'
23505 5' -53.2 NC_005259.1 + 40122 0.66 0.863985
Target:  5'- cGCuCGUGGaaUUGGCGGGCGAGCUCGc-- -3'
miRNA:   3'- -UG-GCACU--AGCUGUUUGCUCGGGUcuu -5'
23505 5' -53.2 NC_005259.1 + 64420 0.66 0.863985
Target:  5'- aGCCGUGAggGAUcgccGACGAGCugCCGGGg -3'
miRNA:   3'- -UGGCACUagCUGu---UUGCUCG--GGUCUu -5'
23505 5' -53.2 NC_005259.1 + 17813 0.66 0.835681
Target:  5'- gGCCgGUGAgcucagcgaggccaUCGACGggUGGGCCgAGGc -3'
miRNA:   3'- -UGG-CACU--------------AGCUGUuuGCUCGGgUCUu -5'
23505 5' -53.2 NC_005259.1 + 51160 0.66 0.829361
Target:  5'- uUCG-GGUUGACcguGGCGAGCCaCAGAu -3'
miRNA:   3'- uGGCaCUAGCUGu--UUGCUCGG-GUCUu -5'
23505 5' -53.2 NC_005259.1 + 35965 0.67 0.791373
Target:  5'- gACCGUGGUCGcugucaacgGCGggaaaccgacGGCGAGgCCGGAc -3'
miRNA:   3'- -UGGCACUAGC---------UGU----------UUGCUCgGGUCUu -5'
23505 5' -53.2 NC_005259.1 + 49443 0.68 0.740183
Target:  5'- cGCCGccuUGGUCGGCAGcCGGauGCCCAGc- -3'
miRNA:   3'- -UGGC---ACUAGCUGUUuGCU--CGGGUCuu -5'
23505 5' -53.2 NC_005259.1 + 59307 0.68 0.729565
Target:  5'- gUCGUGG-CGGCAGcgGCGAGCUCAGc- -3'
miRNA:   3'- uGGCACUaGCUGUU--UGCUCGGGUCuu -5'
23505 5' -53.2 NC_005259.1 + 17379 0.68 0.717772
Target:  5'- gUCGUGGUCGACu-GCGAcacugccGCCCAGc- -3'
miRNA:   3'- uGGCACUAGCUGuuUGCU-------CGGGUCuu -5'
23505 5' -53.2 NC_005259.1 + 9358 0.69 0.675226
Target:  5'- gACCGUcuguaGGUCGuACucGGCGAGCUCGGAGa -3'
miRNA:   3'- -UGGCA-----CUAGC-UGu-UUGCUCGGGUCUU- -5'
23505 5' -53.2 NC_005259.1 + 57062 0.71 0.59769
Target:  5'- cACCGUGAUCGGguCGAACGcGUCgAGGAu -3'
miRNA:   3'- -UGGCACUAGCU--GUUUGCuCGGgUCUU- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.