Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23505 | 5' | -53.2 | NC_005259.1 | + | 57062 | 0.71 | 0.59769 |
Target: 5'- cACCGUGAUCGGguCGAACGcGUCgAGGAu -3' miRNA: 3'- -UGGCACUAGCU--GUUUGCuCGGgUCUU- -5' |
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23505 | 5' | -53.2 | NC_005259.1 | + | 9358 | 0.69 | 0.675226 |
Target: 5'- gACCGUcuguaGGUCGuACucGGCGAGCUCGGAGa -3' miRNA: 3'- -UGGCA-----CUAGC-UGu-UUGCUCGGGUCUU- -5' |
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23505 | 5' | -53.2 | NC_005259.1 | + | 17379 | 0.68 | 0.717772 |
Target: 5'- gUCGUGGUCGACu-GCGAcacugccGCCCAGc- -3' miRNA: 3'- uGGCACUAGCUGuuUGCU-------CGGGUCuu -5' |
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23505 | 5' | -53.2 | NC_005259.1 | + | 59307 | 0.68 | 0.729565 |
Target: 5'- gUCGUGG-CGGCAGcgGCGAGCUCAGc- -3' miRNA: 3'- uGGCACUaGCUGUU--UGCUCGGGUCuu -5' |
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23505 | 5' | -53.2 | NC_005259.1 | + | 49443 | 0.68 | 0.740183 |
Target: 5'- cGCCGccuUGGUCGGCAGcCGGauGCCCAGc- -3' miRNA: 3'- -UGGC---ACUAGCUGUUuGCU--CGGGUCuu -5' |
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23505 | 5' | -53.2 | NC_005259.1 | + | 35965 | 0.67 | 0.791373 |
Target: 5'- gACCGUGGUCGcugucaacgGCGggaaaccgacGGCGAGgCCGGAc -3' miRNA: 3'- -UGGCACUAGC---------UGU----------UUGCUCgGGUCUu -5' |
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23505 | 5' | -53.2 | NC_005259.1 | + | 51160 | 0.66 | 0.829361 |
Target: 5'- uUCG-GGUUGACcguGGCGAGCCaCAGAu -3' miRNA: 3'- uGGCaCUAGCUGu--UUGCUCGG-GUCUu -5' |
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23505 | 5' | -53.2 | NC_005259.1 | + | 17813 | 0.66 | 0.835681 |
Target: 5'- gGCCgGUGAgcucagcgaggccaUCGACGggUGGGCCgAGGc -3' miRNA: 3'- -UGG-CACU--------------AGCUGUuuGCUCGGgUCUu -5' |
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23505 | 5' | -53.2 | NC_005259.1 | + | 51276 | 0.66 | 0.863985 |
Target: 5'- cACgGUGcgCGGCAAGCGGcagcGCCCGa-- -3' miRNA: 3'- -UGgCACuaGCUGUUUGCU----CGGGUcuu -5' |
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23505 | 5' | -53.2 | NC_005259.1 | + | 40122 | 0.66 | 0.863985 |
Target: 5'- cGCuCGUGGaaUUGGCGGGCGAGCUCGc-- -3' miRNA: 3'- -UG-GCACU--AGCUGUUUGCUCGGGUcuu -5' |
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23505 | 5' | -53.2 | NC_005259.1 | + | 64420 | 0.66 | 0.863985 |
Target: 5'- aGCCGUGAggGAUcgccGACGAGCugCCGGGg -3' miRNA: 3'- -UGGCACUagCUGu---UUGCUCG--GGUCUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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