Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23507 | 3' | -54.2 | NC_005259.1 | + | 50269 | 0.66 | 0.78491 |
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Target: 5'- aCUCgcuGUCGUCGG-UGGCgggaacGUCGUGCa- -3' miRNA: 3'- -GAG---UAGCGGCUaACCGa-----CAGCACGag -5' |
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| 23507 | 3' | -54.2 | NC_005259.1 | + | 51409 | 0.67 | 0.774961 |
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Target: 5'- -aCAUCGCCGAggGGCcgGUUGaGgUCu -3' miRNA: 3'- gaGUAGCGGCUaaCCGa-CAGCaCgAG- -5' |
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| 23507 | 3' | -54.2 | NC_005259.1 | + | 1406 | 0.67 | 0.733812 |
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Target: 5'- uCUCAUaCGacCCGAgcacGGCauacGUCGUGCUCg -3' miRNA: 3'- -GAGUA-GC--GGCUaa--CCGa---CAGCACGAG- -5' |
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| 23507 | 3' | -54.2 | NC_005259.1 | + | 41940 | 0.68 | 0.701842 |
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Target: 5'- -aCGUCGCuUGGUUGuGCUGcCGUccGCUCg -3' miRNA: 3'- gaGUAGCG-GCUAAC-CGACaGCA--CGAG- -5' |
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| 23507 | 3' | -54.2 | NC_005259.1 | + | 63382 | 0.7 | 0.593593 |
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Target: 5'- cCUCGUCGCCGA-UGGCccacgccgaccaaugGUUGcccuUGCUCa -3' miRNA: 3'- -GAGUAGCGGCUaACCGa--------------CAGC----ACGAG- -5' |
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| 23507 | 3' | -54.2 | NC_005259.1 | + | 17083 | 0.73 | 0.408109 |
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Target: 5'- gUCAggcCGCCGAUauugGGCUGUCGaucaUGUUCa -3' miRNA: 3'- gAGUa--GCGGCUAa---CCGACAGC----ACGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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