Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23508 | 5' | -64.5 | NC_005259.1 | + | 32490 | 0.68 | 0.264613 |
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Target: 5'- cGCGAGGUGGUucuguaugaacaCCGuGagguaGUCGAGGCg -3' miRNA: 3'- uCGCUCCACCG------------GGC-Cgag--CGGCUCCG- -5' |
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| 23508 | 5' | -64.5 | NC_005259.1 | + | 7683 | 0.68 | 0.262739 |
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Target: 5'- cGUGGGGUGGCCCGuacGUcugcaccaccaucgUCuggGCCGAGGg -3' miRNA: 3'- uCGCUCCACCGGGC---CG--------------AG---CGGCUCCg -5' |
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| 23508 | 5' | -64.5 | NC_005259.1 | + | 25941 | 0.68 | 0.25232 |
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Target: 5'- gAGCGcacgccGGU-GCCCGGCcCGCCGAcGCc -3' miRNA: 3'- -UCGCu-----CCAcCGGGCCGaGCGGCUcCG- -5' |
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| 23508 | 5' | -64.5 | NC_005259.1 | + | 61603 | 0.68 | 0.245762 |
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Target: 5'- cGGUGGGGUcGGCggggaacagcagaCCGGCacCGCCGAcGGUg -3' miRNA: 3'- -UCGCUCCA-CCG-------------GGCCGa-GCGGCU-CCG- -5' |
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| 23508 | 5' | -64.5 | NC_005259.1 | + | 53588 | 0.68 | 0.229148 |
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Target: 5'- cGUGAGGUGuuCCuuuuuGCUCGUCGGGGUc -3' miRNA: 3'- uCGCUCCACcgGGc----CGAGCGGCUCCG- -5' |
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| 23508 | 5' | -64.5 | NC_005259.1 | + | 23347 | 0.69 | 0.218251 |
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Target: 5'- gGGCucGAGGcuGCCUgcgaGGC-CGCCGAGGCa -3' miRNA: 3'- -UCG--CUCCacCGGG----CCGaGCGGCUCCG- -5' |
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| 23508 | 5' | -64.5 | NC_005259.1 | + | 37760 | 0.69 | 0.212971 |
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Target: 5'- cAGCGcGGUGGCCCcuGCggGCaCGAGcGCa -3' miRNA: 3'- -UCGCuCCACCGGGc-CGagCG-GCUC-CG- -5' |
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| 23508 | 5' | -64.5 | NC_005259.1 | + | 63860 | 0.79 | 0.036688 |
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Target: 5'- cGGCGAGGUGG-CCGGCUgGCCuugcguGGGCg -3' miRNA: 3'- -UCGCUCCACCgGGCCGAgCGGc-----UCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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