Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23508 | 5' | -64.5 | NC_005259.1 | + | 65430 | 0.7 | 0.171955 |
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Target: 5'- aAGCGA--UGGCCUGaGCUCGCaccugcgaacaacaaCGAGGCc -3' miRNA: 3'- -UCGCUccACCGGGC-CGAGCG---------------GCUCCG- -5' |
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| 23508 | 5' | -64.5 | NC_005259.1 | + | 56701 | 0.71 | 0.142463 |
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Target: 5'- uGCGAGGUGGCCacgauGGUgUCcCCGAGcGCg -3' miRNA: 3'- uCGCUCCACCGGg----CCG-AGcGGCUC-CG- -5' |
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| 23508 | 5' | -64.5 | NC_005259.1 | + | 54704 | 0.71 | 0.142463 |
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Target: 5'- aGGCG-GGUGGCCUcagccugguacaGGacauaGCCGAGGCu -3' miRNA: 3'- -UCGCuCCACCGGG------------CCgag--CGGCUCCG- -5' |
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| 23508 | 5' | -64.5 | NC_005259.1 | + | 30816 | 0.73 | 0.10164 |
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Target: 5'- gAGUcGGGUaGUCCGGCgCGCCGAGGUc -3' miRNA: 3'- -UCGcUCCAcCGGGCCGaGCGGCUCCG- -5' |
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| 23508 | 5' | -64.5 | NC_005259.1 | + | 20944 | 0.75 | 0.083159 |
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Target: 5'- gGGUGGGGUGGCacgucuacuacacgCGGUaUCGCCGGGGUg -3' miRNA: 3'- -UCGCUCCACCGg-------------GCCG-AGCGGCUCCG- -5' |
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| 23508 | 5' | -64.5 | NC_005259.1 | + | 2104 | 0.76 | 0.068276 |
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Target: 5'- cGGCGAGGccaauUGGCacacGCUCGCCGAGGUc -3' miRNA: 3'- -UCGCUCC-----ACCGggc-CGAGCGGCUCCG- -5' |
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| 23508 | 5' | -64.5 | NC_005259.1 | + | 50374 | 0.76 | 0.063003 |
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Target: 5'- cGGCGAGaaacucGGCCCacgugucgcGGCUCGCCGAGcGCg -3' miRNA: 3'- -UCGCUCca----CCGGG---------CCGAGCGGCUC-CG- -5' |
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| 23508 | 5' | -64.5 | NC_005259.1 | + | 63860 | 0.79 | 0.036688 |
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Target: 5'- cGGCGAGGUGG-CCGGCUgGCCuugcguGGGCg -3' miRNA: 3'- -UCGCUCCACCgGGCCGAgCGGc-----UCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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