Results 61 - 63 of 63 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23511 | 3' | -54.8 | NC_005259.1 | + | 66863 | 0.66 | 0.801089 |
Target: 5'- aCCGaUCGGCcuuguUCGc-ACCGCGCCACg- -3' miRNA: 3'- -GGC-AGCUGu----AGCcaUGGCGUGGUGag -5' |
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23511 | 3' | -54.8 | NC_005259.1 | + | 67856 | 0.79 | 0.194358 |
Target: 5'- -gGUCGACcaAUCGGUGCCGUAggucucgauCCACUCg -3' miRNA: 3'- ggCAGCUG--UAGCCAUGGCGU---------GGUGAG- -5' |
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23511 | 3' | -54.8 | NC_005259.1 | + | 68546 | 0.66 | 0.810367 |
Target: 5'- gCGUCGACAUCGGggugGuuGaGCCAaccgagguggacCUCg -3' miRNA: 3'- gGCAGCUGUAGCCa---UggCgUGGU------------GAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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