Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23512 | 3' | -58.3 | NC_005259.1 | + | 23287 | 0.77 | 0.149106 |
Target: 5'- gGGCGCGGUGGuUGggUAUCggGGUCCGg -3' miRNA: 3'- -CCGCGCCACC-GCuaGUGGuaCCAGGU- -5' |
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23512 | 3' | -58.3 | NC_005259.1 | + | 58938 | 0.74 | 0.220149 |
Target: 5'- gGGCGCGGUGGCagcGUCGgCCuUGG-CCAg -3' miRNA: 3'- -CCGCGCCACCGc--UAGU-GGuACCaGGU- -5' |
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23512 | 3' | -58.3 | NC_005259.1 | + | 2038 | 0.74 | 0.220707 |
Target: 5'- aGUGCGGUgGGCGGUCACCGgcaccgagacacggcGGUUCAa -3' miRNA: 3'- cCGCGCCA-CCGCUAGUGGUa--------------CCAGGU- -5' |
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23512 | 3' | -58.3 | NC_005259.1 | + | 45488 | 0.71 | 0.341272 |
Target: 5'- uGGcCGCuGGUGGCcuuGGUCGCUGUGG-CCAc -3' miRNA: 3'- -CC-GCG-CCACCG---CUAGUGGUACCaGGU- -5' |
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23512 | 3' | -58.3 | NC_005259.1 | + | 57029 | 0.7 | 0.391121 |
Target: 5'- cGGCGCGGUGG-GGUCGCacucguCGUaGGcCCAc -3' miRNA: 3'- -CCGCGCCACCgCUAGUG------GUA-CCaGGU- -5' |
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23512 | 3' | -58.3 | NC_005259.1 | + | 35670 | 0.7 | 0.408761 |
Target: 5'- aGCGCGGUGGUGGUgcacagcgaCGCCGUGc-CCGg -3' miRNA: 3'- cCGCGCCACCGCUA---------GUGGUACcaGGU- -5' |
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23512 | 3' | -58.3 | NC_005259.1 | + | 41501 | 0.69 | 0.445485 |
Target: 5'- --gGCGGUGGCGggCagACCGUGGaUCUg -3' miRNA: 3'- ccgCGCCACCGCuaG--UGGUACC-AGGu -5' |
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23512 | 3' | -58.3 | NC_005259.1 | + | 27996 | 0.69 | 0.43613 |
Target: 5'- uGGUGCGGgugccggGGCGGggUCGCCggGGUUg- -3' miRNA: 3'- -CCGCGCCa------CCGCU--AGUGGuaCCAGgu -5' |
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23512 | 3' | -58.3 | NC_005259.1 | + | 28241 | 0.69 | 0.417765 |
Target: 5'- cGGCGgGGUGGuCGAUCACgA---UCCAu -3' miRNA: 3'- -CCGCgCCACC-GCUAGUGgUaccAGGU- -5' |
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23512 | 3' | -58.3 | NC_005259.1 | + | 1311 | 0.69 | 0.417765 |
Target: 5'- -aCGCGGUcGUGAgCGCCAUGGUCg- -3' miRNA: 3'- ccGCGCCAcCGCUaGUGGUACCAGgu -5' |
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23512 | 3' | -58.3 | NC_005259.1 | + | 54083 | 0.68 | 0.493856 |
Target: 5'- aGGU-CGGUGGCGucgcgcAUCACCggGGUCa- -3' miRNA: 3'- -CCGcGCCACCGC------UAGUGGuaCCAGgu -5' |
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23512 | 3' | -58.3 | NC_005259.1 | + | 36531 | 0.67 | 0.544488 |
Target: 5'- cGCGCGGUgugacacgcggGGUGAUCugCgguGUGGgauUCCAg -3' miRNA: 3'- cCGCGCCA-----------CCGCUAGugG---UACC---AGGU- -5' |
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23512 | 3' | -58.3 | NC_005259.1 | + | 24058 | 0.67 | 0.553792 |
Target: 5'- cGGCGCGGUGcucaaucucguGCaccgcgaGAUCACCGgcagGG-CCAa -3' miRNA: 3'- -CCGCGCCAC-----------CG-------CUAGUGGUa---CCaGGU- -5' |
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23512 | 3' | -58.3 | NC_005259.1 | + | 20064 | 0.67 | 0.554829 |
Target: 5'- cGCuGCGGUGGCGGucgUCACCAc-GUCgAa -3' miRNA: 3'- cCG-CGCCACCGCU---AGUGGUacCAGgU- -5' |
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23512 | 3' | -58.3 | NC_005259.1 | + | 24300 | 0.67 | 0.586163 |
Target: 5'- uGGUGCuGcUGcGCGAUCuACCcgacAUGGUCCGg -3' miRNA: 3'- -CCGCGcC-AC-CGCUAG-UGG----UACCAGGU- -5' |
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23512 | 3' | -58.3 | NC_005259.1 | + | 8952 | 0.66 | 0.596689 |
Target: 5'- gGGCGCGccGUGGaCGggCACCGUgccgggacguaGGUgCCAg -3' miRNA: 3'- -CCGCGC--CACC-GCuaGUGGUA-----------CCA-GGU- -5' |
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23512 | 3' | -58.3 | NC_005259.1 | + | 23313 | 0.66 | 0.63899 |
Target: 5'- nGGCGCGGUcGGUG-UCGCCGag--CCGg -3' miRNA: 3'- -CCGCGCCA-CCGCuAGUGGUaccaGGU- -5' |
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23512 | 3' | -58.3 | NC_005259.1 | + | 55340 | 0.66 | 0.63899 |
Target: 5'- cGGCaGCGGUGGCGAU-GCCGaacgCCu -3' miRNA: 3'- -CCG-CGCCACCGCUAgUGGUaccaGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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