Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23514 | 5' | -57.8 | NC_005259.1 | + | 60568 | 0.72 | 0.334972 |
Target: 5'- -gUGCCGACGGUCaggcGGCCcACGgCGAg -3' miRNA: 3'- gaGCGGCUGCCAGaa--CCGG-UGCaGCU- -5' |
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23514 | 5' | -57.8 | NC_005259.1 | + | 60294 | 0.67 | 0.608069 |
Target: 5'- aUCGUCGuCGGUgc-GGCCACGgacggCGAa -3' miRNA: 3'- gAGCGGCuGCCAgaaCCGGUGCa----GCU- -5' |
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23514 | 5' | -57.8 | NC_005259.1 | + | 58983 | 0.7 | 0.428295 |
Target: 5'- gUCGgCGACGGcgagCUUGGCCuuuucacCGUUGAg -3' miRNA: 3'- gAGCgGCUGCCa---GAACCGGu------GCAGCU- -5' |
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23514 | 5' | -57.8 | NC_005259.1 | + | 58913 | 0.73 | 0.304926 |
Target: 5'- gCUCGUCGGCGGUCUUGu---CGUCGGc -3' miRNA: 3'- -GAGCGGCUGCCAGAACcgguGCAGCU- -5' |
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23514 | 5' | -57.8 | NC_005259.1 | + | 58836 | 0.67 | 0.608069 |
Target: 5'- cCUCGCCGcccuCGGUCUcacccucgGGCuCGcCGUCGu -3' miRNA: 3'- -GAGCGGCu---GCCAGAa-------CCG-GU-GCAGCu -5' |
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23514 | 5' | -57.8 | NC_005259.1 | + | 52921 | 0.66 | 0.639685 |
Target: 5'- -cCGCCGAC--UCgaUGGCCugGUCGc -3' miRNA: 3'- gaGCGGCUGccAGa-ACCGGugCAGCu -5' |
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23514 | 5' | -57.8 | NC_005259.1 | + | 52608 | 0.68 | 0.555855 |
Target: 5'- -gCGCCGuACGGU---GGCCaacuACGUCGAg -3' miRNA: 3'- gaGCGGC-UGCCAgaaCCGG----UGCAGCU- -5' |
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23514 | 5' | -57.8 | NC_005259.1 | + | 49748 | 0.69 | 0.485241 |
Target: 5'- gCUCGUCaguGACGGUCUU-GCCgucuGCGUUGAa -3' miRNA: 3'- -GAGCGG---CUGCCAGAAcCGG----UGCAGCU- -5' |
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23514 | 5' | -57.8 | NC_005259.1 | + | 46611 | 0.72 | 0.334195 |
Target: 5'- gUCGuuGGCGGUCUUGGUggccugaCugGUCa- -3' miRNA: 3'- gAGCggCUGCCAGAACCG-------GugCAGcu -5' |
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23514 | 5' | -57.8 | NC_005259.1 | + | 46554 | 0.66 | 0.650223 |
Target: 5'- gUCGCCGACcgcgccuauGGUC---GCgGCGUCGAc -3' miRNA: 3'- gAGCGGCUG---------CCAGaacCGgUGCAGCU- -5' |
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23514 | 5' | -57.8 | NC_005259.1 | + | 43415 | 0.72 | 0.342814 |
Target: 5'- cCUCGgCGGCGGUUUccucgcucgacUGGCUGCGcUCGAu -3' miRNA: 3'- -GAGCgGCUGCCAGA-----------ACCGGUGC-AGCU- -5' |
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23514 | 5' | -57.8 | NC_005259.1 | + | 42941 | 0.77 | 0.162769 |
Target: 5'- cCUCGCCGugGG-CgaGGCCGCcguuGUCGAa -3' miRNA: 3'- -GAGCGGCugCCaGaaCCGGUG----CAGCU- -5' |
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23514 | 5' | -57.8 | NC_005259.1 | + | 42769 | 0.67 | 0.576622 |
Target: 5'- -cUGCuCGGCGGUCUcggGGCCGCcacCGAu -3' miRNA: 3'- gaGCG-GCUGCCAGAa--CCGGUGca-GCU- -5' |
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23514 | 5' | -57.8 | NC_005259.1 | + | 39900 | 0.74 | 0.238379 |
Target: 5'- gUCGCCGcCGcccgccucgauccGUCgagGGCCGCGUCGAg -3' miRNA: 3'- gAGCGGCuGC-------------CAGaa-CCGGUGCAGCU- -5' |
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23514 | 5' | -57.8 | NC_005259.1 | + | 38655 | 0.68 | 0.566214 |
Target: 5'- aCUCGCCGAaaucgaccaccuCGcUgUUGGCCGCcggGUCGAg -3' miRNA: 3'- -GAGCGGCU------------GCcAgAACCGGUG---CAGCU- -5' |
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23514 | 5' | -57.8 | NC_005259.1 | + | 38064 | 0.66 | 0.681709 |
Target: 5'- -cUGCCGACGaGccgccaUCgagcaUGGcCCACGUCGAg -3' miRNA: 3'- gaGCGGCUGC-C------AGa----ACC-GGUGCAGCU- -5' |
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23514 | 5' | -57.8 | NC_005259.1 | + | 36442 | 0.72 | 0.342814 |
Target: 5'- gUCGCCGA-GGUCga--CCGCGUCGAg -3' miRNA: 3'- gAGCGGCUgCCAGaaccGGUGCAGCU- -5' |
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23514 | 5' | -57.8 | NC_005259.1 | + | 33902 | 0.66 | 0.669147 |
Target: 5'- -cCGCgCGACGGgccgagaccGGCCACGgugCGAg -3' miRNA: 3'- gaGCG-GCUGCCagaa-----CCGGUGCa--GCU- -5' |
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23514 | 5' | -57.8 | NC_005259.1 | + | 28963 | 0.66 | 0.671244 |
Target: 5'- aUCGCCGA-GGUCggugccgaGGCCGCGagCGc -3' miRNA: 3'- gAGCGGCUgCCAGaa------CCGGUGCa-GCu -5' |
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23514 | 5' | -57.8 | NC_005259.1 | + | 26506 | 0.7 | 0.450612 |
Target: 5'- gCUCGCCGcccggcgagaagagcGCGGUgagcagCUUGGCCACcaCGAu -3' miRNA: 3'- -GAGCGGC---------------UGCCA------GAACCGGUGcaGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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