miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23515 3' -60 NC_005259.1 + 13432 0.67 0.496306
Target:  5'- -cGcCGGGgccgCgcuagGCGUGACCgUCGGCGcGg -3'
miRNA:   3'- caC-GCCCa---Ga----CGCACUGG-AGCCGCuC- -5'
23515 3' -60 NC_005259.1 + 64791 0.67 0.467144
Target:  5'- cUGCGacuGG-CUGUGUGccucgGCCUCGGCGGc -3'
miRNA:   3'- cACGC---CCaGACGCAC-----UGGAGCCGCUc -5'
23515 3' -60 NC_005259.1 + 2083 0.68 0.429692
Target:  5'- -gGCGGGcCUGCucGAUUucaUCGGCGAGg -3'
miRNA:   3'- caCGCCCaGACGcaCUGG---AGCCGCUC- -5'
23515 3' -60 NC_005259.1 + 65279 0.68 0.394065
Target:  5'- uGUGUGGGUgCUGcCGUGGauCUUCGGCaAGg -3'
miRNA:   3'- -CACGCCCA-GAC-GCACU--GGAGCCGcUC- -5'
23515 3' -60 NC_005259.1 + 63936 0.71 0.292255
Target:  5'- -cGUGGGUCUuaccguccggcaGCGaGAUCUCGGCGGu -3'
miRNA:   3'- caCGCCCAGA------------CGCaCUGGAGCCGCUc -5'
23515 3' -60 NC_005259.1 + 58784 0.73 0.206892
Target:  5'- uGUGCGccGGUCgagGCgGUGauuGCCUCGGCGGGc -3'
miRNA:   3'- -CACGC--CCAGa--CG-CAC---UGGAGCCGCUC- -5'
23515 3' -60 NC_005259.1 + 41642 0.78 0.091241
Target:  5'- gGUGCcGGUCUGCG-GGCgCUCGGUGAGc -3'
miRNA:   3'- -CACGcCCAGACGCaCUG-GAGCCGCUC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.