Results 41 - 53 of 53 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23516 | 5' | -55.6 | NC_005259.1 | + | 47691 | 0.66 | 0.732905 |
Target: 5'- uGCA-UGCccGGUuggauGCGCG-GCAGUGCCu -3' miRNA: 3'- -CGUcACGcuUCG-----UGCGCuCGUCACGG- -5' |
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23516 | 5' | -55.6 | NC_005259.1 | + | 27395 | 0.66 | 0.743257 |
Target: 5'- gGCGGUGuCGGcAGCG-GCGgcAGCucGGUGCCc -3' miRNA: 3'- -CGUCAC-GCU-UCGUgCGC--UCG--UCACGG- -5' |
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23516 | 5' | -55.6 | NC_005259.1 | + | 17273 | 0.66 | 0.745314 |
Target: 5'- gGCAGUGCucgaccgcuauuuGAAGCgguacuugacccuguACGcCGAGCGGUaccccgaggguguGCCg -3' miRNA: 3'- -CGUCACG-------------CUUCG---------------UGC-GCUCGUCA-------------CGG- -5' |
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23516 | 5' | -55.6 | NC_005259.1 | + | 65317 | 0.66 | 0.753499 |
Target: 5'- aGCuGUGCGAAGaguucgAUGuCGAGCugcuGUGCg -3' miRNA: 3'- -CGuCACGCUUCg-----UGC-GCUCGu---CACGg -5' |
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23516 | 5' | -55.6 | NC_005259.1 | + | 66255 | 0.66 | 0.76362 |
Target: 5'- cGCAGcGCGGccCGCGcCGGGCGGcgGCUc -3' miRNA: 3'- -CGUCaCGCUucGUGC-GCUCGUCa-CGG- -5' |
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23516 | 5' | -55.6 | NC_005259.1 | + | 43063 | 0.66 | 0.76362 |
Target: 5'- aCGGggucgGUGAGGaACGCGAGCccguUGCCg -3' miRNA: 3'- cGUCa----CGCUUCgUGCGCUCGuc--ACGG- -5' |
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23516 | 5' | -55.6 | NC_005259.1 | + | 41897 | 0.66 | 0.76362 |
Target: 5'- cGCA-UGCGGgaugAGCGCGgGAGCucgaaGGUGaCCc -3' miRNA: 3'- -CGUcACGCU----UCGUGCgCUCG-----UCAC-GG- -5' |
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23516 | 5' | -55.6 | NC_005259.1 | + | 31226 | 0.66 | 0.76362 |
Target: 5'- uCAGUcCGAugagaccauuGgGCGCG-GCGGUGCCg -3' miRNA: 3'- cGUCAcGCUu---------CgUGCGCuCGUCACGG- -5' |
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23516 | 5' | -55.6 | NC_005259.1 | + | 2928 | 0.66 | 0.76362 |
Target: 5'- cGgAGUaGCGAaagcguccaaGGCGCGCGAGacccugGCCa -3' miRNA: 3'- -CgUCA-CGCU----------UCGUGCGCUCguca--CGG- -5' |
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23516 | 5' | -55.6 | NC_005259.1 | + | 27994 | 0.66 | 0.76362 |
Target: 5'- gGUGGUGCGGGuGC-CG-GGGCGGggucGCCg -3' miRNA: 3'- -CGUCACGCUU-CGuGCgCUCGUCa---CGG- -5' |
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23516 | 5' | -55.6 | NC_005259.1 | + | 27151 | 0.66 | 0.770628 |
Target: 5'- uGCGGUGuUGggGCcaacgaugccgucgGCGaUGAGCAG-GCUg -3' miRNA: 3'- -CGUCAC-GCuuCG--------------UGC-GCUCGUCaCGG- -5' |
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23516 | 5' | -55.6 | NC_005259.1 | + | 11128 | 0.66 | 0.772618 |
Target: 5'- aCGGUGCGAgccgucgccugcgGGUAacacuucggUGCG-GCAGUGCUu -3' miRNA: 3'- cGUCACGCU-------------UCGU---------GCGCuCGUCACGG- -5' |
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23516 | 5' | -55.6 | NC_005259.1 | + | 47037 | 0.66 | 0.773611 |
Target: 5'- aGCAG-GcCGggGaucuGCGCGAGC-GUGUUg -3' miRNA: 3'- -CGUCaC-GCuuCg---UGCGCUCGuCACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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