Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23519 | 5' | -47.9 | NC_005260.1 | + | 28004 | 0.66 | 0.999904 |
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Target: 5'- uGGAGGUGCGGUUGcAUUGCaugaGCCGu- -3' miRNA: 3'- uCCUUUACGCUAACuUAGUG----CGGUga -5' |
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| 23519 | 5' | -47.9 | NC_005260.1 | + | 114859 | 0.67 | 0.999875 |
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Target: 5'- aAGGcAGAUGCGcuauccuGUUGCGCCACa -3' miRNA: 3'- -UCC-UUUACGCuaacu--UAGUGCGGUGa -5' |
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| 23519 | 5' | -47.9 | NC_005260.1 | + | 131405 | 0.67 | 0.999668 |
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Target: 5'- uAGGuAAGUGCGAUUGAuAUCAaCGCa--- -3' miRNA: 3'- -UCC-UUUACGCUAACU-UAGU-GCGguga -5' |
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| 23519 | 5' | -47.9 | NC_005260.1 | + | 101312 | 0.67 | 0.999668 |
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Target: 5'- cGGAAGgcaCGAUUGGAUCACGUa--- -3' miRNA: 3'- uCCUUUac-GCUAACUUAGUGCGguga -5' |
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| 23519 | 5' | -47.9 | NC_005260.1 | + | 140206 | 0.69 | 0.998815 |
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Target: 5'- --cAGAUGCGAUUGGAa-GCGUCGCUg -3' miRNA: 3'- uccUUUACGCUAACUUagUGCGGUGA- -5' |
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| 23519 | 5' | -47.9 | NC_005260.1 | + | 160307 | 0.69 | 0.998271 |
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Target: 5'- -uGGAAUGCGuUUGGAUUACGUUGCa -3' miRNA: 3'- ucCUUUACGCuAACUUAGUGCGGUGa -5' |
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| 23519 | 5' | -47.9 | NC_005260.1 | + | 143881 | 0.71 | 0.993589 |
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Target: 5'- cGGAAGaGUGAUUGGuuUCACGCCucCUg -3' miRNA: 3'- uCCUUUaCGCUAACUu-AGUGCGGu-GA- -5' |
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| 23519 | 5' | -47.9 | NC_005260.1 | + | 20082 | 1.08 | 0.026909 |
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Target: 5'- gAGGAAAUGCGAUUGAAUCACGCCACUc -3' miRNA: 3'- -UCCUUUACGCUAACUUAGUGCGGUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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