Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2352 | 3' | -59.3 | NC_001416.1 | + | 18356 | 0.66 | 0.440613 |
Target: 5'- cCGCUGGUaauggugGgGGcCGUCaGGC-GCUUCAGg -3' miRNA: 3'- -GCGACCG-------CgUC-GCAG-CCGuCGAAGUC- -5' |
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2352 | 3' | -59.3 | NC_001416.1 | + | 17242 | 1.07 | 0.000406 |
Target: 5'- uCGCUGGCGCAGCGUCGGCAGCUUCAGc -3' miRNA: 3'- -GCGACCGCGUCGCAGCCGUCGAAGUC- -5' |
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2352 | 3' | -59.3 | NC_001416.1 | + | 16855 | 0.67 | 0.400304 |
Target: 5'- uGCUGGaaaCGCagaccguggauuucAGCGUCGGCgcagaagGGCUUCGc -3' miRNA: 3'- gCGACC---GCG--------------UCGCAGCCG-------UCGAAGUc -5' |
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2352 | 3' | -59.3 | NC_001416.1 | + | 12669 | 0.69 | 0.303272 |
Target: 5'- gGC-GGCGCAuccGCGUCaGGCGGUacagccaUUCAGg -3' miRNA: 3'- gCGaCCGCGU---CGCAG-CCGUCG-------AAGUC- -5' |
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2352 | 3' | -59.3 | NC_001416.1 | + | 12198 | 0.72 | 0.186372 |
Target: 5'- gCGCUGGCGCAGCa--GGCGGauaaaUUCGc -3' miRNA: 3'- -GCGACCGCGUCGcagCCGUCg----AAGUc -5' |
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2352 | 3' | -59.3 | NC_001416.1 | + | 6753 | 0.73 | 0.158541 |
Target: 5'- cCGCUGGCGUucAGCG-CGuaGGCUUCGa -3' miRNA: 3'- -GCGACCGCG--UCGCaGCcgUCGAAGUc -5' |
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2352 | 3' | -59.3 | NC_001416.1 | + | 3107 | 0.66 | 0.441578 |
Target: 5'- aGCUGGau-GGCGUUGGCGGCa---- -3' miRNA: 3'- gCGACCgcgUCGCAGCCGUCGaaguc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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