Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2352 | 5' | -50.8 | NC_001416.1 | + | 1744 | 0.66 | 0.903104 |
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Target: 5'- aCGCUGUccagauGUgaaaGGUcACACUGUCAGGu -3' miRNA: 3'- -GCGACA------UAag--UCGuUGUGGCAGUCCu -5' |
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| 2352 | 5' | -50.8 | NC_001416.1 | + | 5978 | 0.66 | 0.895885 |
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Target: 5'- cCGCaagaaUGccaaCGGCAGCACCGUCGGuGGu -3' miRNA: 3'- -GCG-----ACauaaGUCGUUGUGGCAGUC-CU- -5' |
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| 2352 | 5' | -50.8 | NC_001416.1 | + | 16535 | 0.66 | 0.880583 |
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Target: 5'- aCGCUGUGUggUCAGguACGCauuaCAGGu -3' miRNA: 3'- -GCGACAUA--AGUCguUGUGgca-GUCCu -5' |
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| 2352 | 5' | -50.8 | NC_001416.1 | + | 17433 | 0.67 | 0.855555 |
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Target: 5'- cCGCUGUcugCAGUGACuucuGCgGUCAGGu -3' miRNA: 3'- -GCGACAuaaGUCGUUG----UGgCAGUCCu -5' |
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| 2352 | 5' | -50.8 | NC_001416.1 | + | 12659 | 0.67 | 0.83758 |
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Target: 5'- gGCUGUuggUGGCGGCGCauccgCGUCAGGc -3' miRNA: 3'- gCGACAuaaGUCGUUGUG-----GCAGUCCu -5' |
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| 2352 | 5' | -50.8 | NC_001416.1 | + | 35133 | 0.67 | 0.83758 |
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Target: 5'- uGUgGUAUUCAGcCAGCACUGUaAGGu -3' miRNA: 3'- gCGaCAUAAGUC-GUUGUGGCAgUCCu -5' |
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| 2352 | 5' | -50.8 | NC_001416.1 | + | 14599 | 0.68 | 0.798887 |
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Target: 5'- gGCUGUAUcaggugccguugUCAGCGGCACaGcCGGGc -3' miRNA: 3'- gCGACAUA------------AGUCGUUGUGgCaGUCCu -5' |
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| 2352 | 5' | -50.8 | NC_001416.1 | + | 17622 | 0.7 | 0.667832 |
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Target: 5'- cCGCac----CGGCAGCACCGUCgAGGAu -3' miRNA: 3'- -GCGacauaaGUCGUUGUGGCAG-UCCU- -5' |
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| 2352 | 5' | -50.8 | NC_001416.1 | + | 8500 | 0.8 | 0.211919 |
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Target: 5'- aCGCcGUGgUCAGCGGCACCGccaUCGGGAu -3' miRNA: 3'- -GCGaCAUaAGUCGUUGUGGC---AGUCCU- -5' |
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| 2352 | 5' | -50.8 | NC_001416.1 | + | 17208 | 1.11 | 0.001812 |
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Target: 5'- aCGCUGUAUUCAGCAACACCGUCAGGAa -3' miRNA: 3'- -GCGACAUAAGUCGUUGUGGCAGUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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