miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23520 3' -48.5 NC_005260.1 + 191726 0.66 0.999939
Target:  5'- gACGauaACUGGCuGAUUCaUUACGUUGUGg -3'
miRNA:   3'- -UGCa--UGGCCG-CUAAG-GAUGCAAUAUg -5'
23520 3' -48.5 NC_005260.1 + 157322 0.68 0.999179
Target:  5'- uCGUGCUGGCugGAUUCCggGCGUa---- -3'
miRNA:   3'- uGCAUGGCCG--CUAAGGa-UGCAauaug -5'
23520 3' -48.5 NC_005260.1 + 467 0.68 0.998784
Target:  5'- aGCGaUAUCGGUGaAUUCCUGCuGUUAg-- -3'
miRNA:   3'- -UGC-AUGGCCGC-UAAGGAUG-CAAUaug -5'
23520 3' -48.5 NC_005260.1 + 49603 0.71 0.988991
Target:  5'- cACGuUACCcGCGAUUCCauUGCGUUcuaAUACa -3'
miRNA:   3'- -UGC-AUGGcCGCUAAGG--AUGCAA---UAUG- -5'
23520 3' -48.5 NC_005260.1 + 49904 1.1 0.020755
Target:  5'- aACGUACCGGCGAUUCCUACGUUAUACu -3'
miRNA:   3'- -UGCAUGGCCGCUAAGGAUGCAAUAUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.