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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23520 | 3' | -48.5 | NC_005260.1 | + | 191726 | 0.66 | 0.999939 |
Target: 5'- gACGauaACUGGCuGAUUCaUUACGUUGUGg -3' miRNA: 3'- -UGCa--UGGCCG-CUAAG-GAUGCAAUAUg -5' |
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23520 | 3' | -48.5 | NC_005260.1 | + | 157322 | 0.68 | 0.999179 |
Target: 5'- uCGUGCUGGCugGAUUCCggGCGUa---- -3' miRNA: 3'- uGCAUGGCCG--CUAAGGa-UGCAauaug -5' |
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23520 | 3' | -48.5 | NC_005260.1 | + | 467 | 0.68 | 0.998784 |
Target: 5'- aGCGaUAUCGGUGaAUUCCUGCuGUUAg-- -3' miRNA: 3'- -UGC-AUGGCCGC-UAAGGAUG-CAAUaug -5' |
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23520 | 3' | -48.5 | NC_005260.1 | + | 49603 | 0.71 | 0.988991 |
Target: 5'- cACGuUACCcGCGAUUCCauUGCGUUcuaAUACa -3' miRNA: 3'- -UGC-AUGGcCGCUAAGG--AUGCAA---UAUG- -5' |
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23520 | 3' | -48.5 | NC_005260.1 | + | 49904 | 1.1 | 0.020755 |
Target: 5'- aACGUACCGGCGAUUCCUACGUUAUACu -3' miRNA: 3'- -UGCAUGGCCGCUAAGGAUGCAAUAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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