Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23520 | 5' | -45.2 | NC_005260.1 | + | 100976 | 0.66 | 1 |
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Target: 5'- uUG-AUGAUAACgGCAAUGuuauccGUCGCGg -3' miRNA: 3'- uACaUACUAUUG-CGUUACcu----UAGCGC- -5' |
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| 23520 | 5' | -45.2 | NC_005260.1 | + | 132743 | 0.66 | 1 |
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Target: 5'- cGUGUucGUGAUAACGCugcuauuguGUGGAgacccgaauugGUUGCa -3' miRNA: 3'- -UACA--UACUAUUGCGu--------UACCU-----------UAGCGc -5' |
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| 23520 | 5' | -45.2 | NC_005260.1 | + | 100300 | 0.66 | 0.999999 |
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Target: 5'- gAUGUGUGcu--CGuCAGUGGGAUgGCa -3' miRNA: 3'- -UACAUACuauuGC-GUUACCUUAgCGc -5' |
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| 23520 | 5' | -45.2 | NC_005260.1 | + | 179731 | 0.67 | 0.999999 |
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Target: 5'- gAUGUAUGAgc-CGcCAAUGGAAUUuaGCu -3' miRNA: 3'- -UACAUACUauuGC-GUUACCUUAG--CGc -5' |
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| 23520 | 5' | -45.2 | NC_005260.1 | + | 143776 | 0.67 | 0.999999 |
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Target: 5'- -gGUAUauucgGGUGGCGC-AUGGcGUCGCa -3' miRNA: 3'- uaCAUA-----CUAUUGCGuUACCuUAGCGc -5' |
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| 23520 | 5' | -45.2 | NC_005260.1 | + | 130266 | 0.66 | 0.999999 |
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Target: 5'- aAUGUAgcguuuaAUGGCGUAGU-GAAUCGCGa -3' miRNA: 3'- -UACAUac-----UAUUGCGUUAcCUUAGCGC- -5' |
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| 23520 | 5' | -45.2 | NC_005260.1 | + | 112766 | 0.67 | 0.999998 |
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Target: 5'- -gGUAuUGAUAACGCucUGGAagauGUCGUc -3' miRNA: 3'- uaCAU-ACUAUUGCGuuACCU----UAGCGc -5' |
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| 23520 | 5' | -45.2 | NC_005260.1 | + | 135007 | 0.67 | 0.999997 |
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Target: 5'- -cGUGcUGAUAaccccaGCGCGAUGGAcuaGCGa -3' miRNA: 3'- uaCAU-ACUAU------UGCGUUACCUuagCGC- -5' |
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| 23520 | 5' | -45.2 | NC_005260.1 | + | 160122 | 0.69 | 0.999907 |
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Target: 5'- -----cGGUAccACGCAGUGGggUCaGCGc -3' miRNA: 3'- uacauaCUAU--UGCGUUACCuuAG-CGC- -5' |
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| 23520 | 5' | -45.2 | NC_005260.1 | + | 49870 | 0.89 | 0.489456 |
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Target: 5'- aAUGUAUuagAACGCAAUGGAAUCGCGg -3' miRNA: 3'- -UACAUAcuaUUGCGUUACCUUAGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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