Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23522 | 5' | -49.7 | NC_005260.1 | + | 21002 | 0.66 | 0.999294 |
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Target: 5'- -aUACCGCGCUCGAUGUgauagcgaUCUuuACCAa -3' miRNA: 3'- aaAUGGCGUGGGUUGUA--------AGAucUGGU- -5' |
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| 23522 | 5' | -49.7 | NC_005260.1 | + | 33004 | 0.66 | 0.999294 |
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Target: 5'- aUUGCCGCACCUAcCAUugcuUCUuu-CCAu -3' miRNA: 3'- aAAUGGCGUGGGUuGUA----AGAucuGGU- -5' |
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| 23522 | 5' | -49.7 | NC_005260.1 | + | 179704 | 0.67 | 0.996863 |
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Target: 5'- -cUGCCGUcucgaACCgGAaguccCGUUCUGGGCCAu -3' miRNA: 3'- aaAUGGCG-----UGGgUU-----GUAAGAUCUGGU- -5' |
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| 23522 | 5' | -49.7 | NC_005260.1 | + | 118605 | 0.68 | 0.99567 |
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Target: 5'- -cUGCCGCACagaCUGACGUUCc-GACCGg -3' miRNA: 3'- aaAUGGCGUG---GGUUGUAAGauCUGGU- -5' |
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| 23522 | 5' | -49.7 | NC_005260.1 | + | 99567 | 0.68 | 0.99567 |
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Target: 5'- cUUUGcCCGCGCCCGACuaacaaUCcgguGACCAa -3' miRNA: 3'- -AAAU-GGCGUGGGUUGua----AGau--CUGGU- -5' |
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| 23522 | 5' | -49.7 | NC_005260.1 | + | 89061 | 0.68 | 0.99567 |
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Target: 5'- -gUACCGUACCCGAUAcUCUGcuACUAu -3' miRNA: 3'- aaAUGGCGUGGGUUGUaAGAUc-UGGU- -5' |
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| 23522 | 5' | -49.7 | NC_005260.1 | + | 166119 | 0.69 | 0.98832 |
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Target: 5'- -aUACCGCACCUcGCAagCgaguGACCGa -3' miRNA: 3'- aaAUGGCGUGGGuUGUaaGau--CUGGU- -5' |
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| 23522 | 5' | -49.7 | NC_005260.1 | + | 69132 | 0.7 | 0.978803 |
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Target: 5'- --cACCGaCACCaauuUGUUCUGGACCAa -3' miRNA: 3'- aaaUGGC-GUGGguu-GUAAGAUCUGGU- -5' |
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| 23522 | 5' | -49.7 | NC_005260.1 | + | 134577 | 1.07 | 0.019213 |
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Target: 5'- uUUUACCGCACCCAACAUUCUAGACCAa -3' miRNA: 3'- -AAAUGGCGUGGGUUGUAAGAUCUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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