Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23523 | 3' | -44.5 | NC_005260.1 | + | 130054 | 0.66 | 1 |
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Target: 5'- aCGGACGUAUUGACgacuCGGa------- -3' miRNA: 3'- -GCCUGCGUAAUUGau--GCCauugaaau -5' |
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| 23523 | 3' | -44.5 | NC_005260.1 | + | 101105 | 0.66 | 1 |
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Target: 5'- aCGGACuGCGUaccGAUUuucuCGGUAACUUg- -3' miRNA: 3'- -GCCUG-CGUAa--UUGAu---GCCAUUGAAau -5' |
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| 23523 | 3' | -44.5 | NC_005260.1 | + | 197121 | 0.67 | 0.999999 |
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Target: 5'- gGGAUGUucauACUACcguuGGUAACUUUGa -3' miRNA: 3'- gCCUGCGuaauUGAUG----CCAUUGAAAU- -5' |
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| 23523 | 3' | -44.5 | NC_005260.1 | + | 30367 | 0.67 | 0.999999 |
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Target: 5'- uCGGAuCGUgagAUUAACUAUGGUGGgUUa- -3' miRNA: 3'- -GCCU-GCG---UAAUUGAUGCCAUUgAAau -5' |
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| 23523 | 3' | -44.5 | NC_005260.1 | + | 87047 | 0.69 | 0.999992 |
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Target: 5'- uGGACGUAU--GCUaggugACGGUAuuuACUUUAg -3' miRNA: 3'- gCCUGCGUAauUGA-----UGCCAU---UGAAAU- -5' |
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| 23523 | 3' | -44.5 | NC_005260.1 | + | 62368 | 0.7 | 0.99996 |
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Target: 5'- aCGGAUGCAcc-GCgACGGUGACa--- -3' miRNA: 3'- -GCCUGCGUaauUGaUGCCAUUGaaau -5' |
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| 23523 | 3' | -44.5 | NC_005260.1 | + | 47159 | 0.7 | 0.999947 |
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Target: 5'- aGGuCGCAUUAcucgauaugaguGCcGCGGUGGCUUg- -3' miRNA: 3'- gCCuGCGUAAU------------UGaUGCCAUUGAAau -5' |
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| 23523 | 3' | -44.5 | NC_005260.1 | + | 93730 | 0.73 | 0.998779 |
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Target: 5'- aGGACGCcgUucacaacGCUGCGGUAgaGCUUg- -3' miRNA: 3'- gCCUGCGuaAu------UGAUGCCAU--UGAAau -5' |
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| 23523 | 3' | -44.5 | NC_005260.1 | + | 113883 | 0.74 | 0.996937 |
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Target: 5'- aUGcGACGUAUgacGACUAUGGUAACUUc- -3' miRNA: 3'- -GC-CUGCGUAa--UUGAUGCCAUUGAAau -5' |
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| 23523 | 3' | -44.5 | NC_005260.1 | + | 152688 | 1.08 | 0.061543 |
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Target: 5'- cCGGACGCAUUAACUACGGUAACUUUAc -3' miRNA: 3'- -GCCUGCGUAAUUGAUGCCAUUGAAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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