Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23524 | 3' | -39.4 | NC_005260.1 | + | 106138 | 0.68 | 1 |
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Target: 5'- ---aUUGUUUGGAgGAGAAAGuGGGAu -3' miRNA: 3'- auuaGAUAAAUCUgCUUUUUU-CCCUc -5' |
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| 23524 | 3' | -39.4 | NC_005260.1 | + | 110380 | 0.68 | 1 |
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Target: 5'- -----aAUUUGGugGAGAAAAuGGGAc -3' miRNA: 3'- auuagaUAAAUCugCUUUUUU-CCCUc -5' |
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| 23524 | 3' | -39.4 | NC_005260.1 | + | 175486 | 0.67 | 1 |
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Target: 5'- gGGUCUAUU---AUGuGAAAAAGGGAGc -3' miRNA: 3'- aUUAGAUAAaucUGC-UUUUUUCCCUC- -5' |
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| 23524 | 3' | -39.4 | NC_005260.1 | + | 32032 | 0.66 | 1 |
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Target: 5'- ---aUUAUUUAGAUaaacAAAAAGGGAGc -3' miRNA: 3'- auuaGAUAAAUCUGcu--UUUUUCCCUC- -5' |
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| 23524 | 3' | -39.4 | NC_005260.1 | + | 8736 | 0.66 | 1 |
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Target: 5'- aGAUCgcgacuGGCGAGAAAAGuGGAu -3' miRNA: 3'- aUUAGauaaauCUGCUUUUUUC-CCUc -5' |
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| 23524 | 3' | -39.4 | NC_005260.1 | + | 41592 | 0.66 | 1 |
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Target: 5'- --cUCUGUucUUGGGCGAAGcaauGGGAu -3' miRNA: 3'- auuAGAUA--AAUCUGCUUUuuu-CCCUc -5' |
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| 23524 | 3' | -39.4 | NC_005260.1 | + | 160365 | 1.11 | 0.155105 |
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Target: 5'- uUAAUCUAUUUAGACGAAAAAAGGGAGa -3' miRNA: 3'- -AUUAGAUAAAUCUGCUUUUUUCCCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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