Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23525 | 3' | -56.2 | NC_005260.1 | + | 8757 | 0.66 | 0.949074 |
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Target: 5'- --aUUGCGGUGGCgaUGCUA-CUaagaUCGCg -3' miRNA: 3'- auaGACGCCACCGg-ACGAUaGA----GGCG- -5' |
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| 23525 | 3' | -56.2 | NC_005260.1 | + | 71709 | 0.66 | 0.940319 |
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Target: 5'- -cUCUGUGGaaacugagUGGCCacauUGCUAa-UCCGCg -3' miRNA: 3'- auAGACGCC--------ACCGG----ACGAUagAGGCG- -5' |
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| 23525 | 3' | -56.2 | NC_005260.1 | + | 122376 | 0.67 | 0.90243 |
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Target: 5'- cAUCUGCGGaGGCaUUGUU-UCUuuGCg -3' miRNA: 3'- aUAGACGCCaCCG-GACGAuAGAggCG- -5' |
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| 23525 | 3' | -56.2 | NC_005260.1 | + | 43944 | 0.67 | 0.90243 |
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Target: 5'- ----cGCGGUGGCgCUGCUA-CUCUu- -3' miRNA: 3'- auagaCGCCACCG-GACGAUaGAGGcg -5' |
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| 23525 | 3' | -56.2 | NC_005260.1 | + | 37267 | 0.67 | 0.896102 |
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Target: 5'- uUAUCU-CGGUGcGCCUGgU-UCUCgCGCa -3' miRNA: 3'- -AUAGAcGCCAC-CGGACgAuAGAG-GCG- -5' |
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| 23525 | 3' | -56.2 | NC_005260.1 | + | 163117 | 1.11 | 0.002919 |
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Target: 5'- gUAUCUGCGGUGGCCUGCUAUCUCCGCg -3' miRNA: 3'- -AUAGACGCCACCGGACGAUAGAGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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