miRNA: 3'- aauuACAAaUGACA-AGCGCAGUcGUU- -5'
miRNA: 3'- aaUUACAAAUG---ACAAGCgc-AGUCGUU- -5'
miRNA: 3'- aauUACAAAUGACAAG-CGCagUCGUU- -5'
miRNA: 3'- -AAUUACAAAUGACAAGCGCAGUCGUU- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23526 | 3' | -45.5 | NC_005260.1 | + | 182497 | 0.66 | 0.999996 |
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Target: 5'- ---uUGUUcGCUGUaUCGCGUCAaCAAg -3' miRNA: 3'- aauuACAAaUGACA-AGCGCAGUcGUU- -5' |
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| 23526 | 3' | -45.5 | NC_005260.1 | + | 65921 | 0.67 | 0.99997 |
|
Target: 5'- -aAGUGUUUGCcgaUGUUCGauuUCAGCAGc -3' miRNA: 3'- aaUUACAAAUG---ACAAGCgc-AGUCGUU- -5' |
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| 23526 | 3' | -45.5 | NC_005260.1 | + | 45868 | 0.69 | 0.999726 |
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Target: 5'- --cAUGUUUACUGUUCuGCG--AGCAAa -3' miRNA: 3'- aauUACAAAUGACAAG-CGCagUCGUU- -5' |
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| 23526 | 3' | -45.5 | NC_005260.1 | + | 182792 | 1.04 | 0.065027 |
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Target: 5'- uUUAAUGUUUACUGUUCGCGUCAGCAAu -3' miRNA: 3'- -AAUUACAAAUGACAAGCGCAGUCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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