Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23529 | 3' | -50 | NC_005260.1 | + | 65552 | 0.66 | 0.999352 |
Target: 5'- aAGCU-GUGCCUg----UGCUUGuGCCa -3' miRNA: 3'- gUCGAcCAUGGAauuugACGAAC-CGG- -5' |
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23529 | 3' | -50 | NC_005260.1 | + | 76889 | 0.66 | 0.999352 |
Target: 5'- aCGGUaccgaacuUGGUAUCuUUGAAUUGCUUGaauucGCCa -3' miRNA: 3'- -GUCG--------ACCAUGG-AAUUUGACGAAC-----CGG- -5' |
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23529 | 3' | -50 | NC_005260.1 | + | 8468 | 0.66 | 0.999352 |
Target: 5'- gCGGCUGGUuuUaUGAGC-GCgaUGGCCa -3' miRNA: 3'- -GUCGACCAugGaAUUUGaCGa-ACCGG- -5' |
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23529 | 3' | -50 | NC_005260.1 | + | 27098 | 0.66 | 0.998816 |
Target: 5'- cCAGCUu-UACCUUGAACUGUUgcGGUa -3' miRNA: 3'- -GUCGAccAUGGAAUUUGACGAa-CCGg -5' |
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23529 | 3' | -50 | NC_005260.1 | + | 185507 | 0.66 | 0.998569 |
Target: 5'- gAGCUGG-AC---GAACUGCUUGcGCg -3' miRNA: 3'- gUCGACCaUGgaaUUUGACGAAC-CGg -5' |
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23529 | 3' | -50 | NC_005260.1 | + | 76310 | 0.67 | 0.998279 |
Target: 5'- uGGCUGcUGCC--AAACUGgcUGGCCa -3' miRNA: 3'- gUCGACcAUGGaaUUUGACgaACCGG- -5' |
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23529 | 3' | -50 | NC_005260.1 | + | 202134 | 0.67 | 0.997099 |
Target: 5'- uCGGCUGGgaaaaACUcgAAACUGCU-GGUa -3' miRNA: 3'- -GUCGACCa----UGGaaUUUGACGAaCCGg -5' |
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23529 | 3' | -50 | NC_005260.1 | + | 190101 | 0.68 | 0.992723 |
Target: 5'- -cGCguuUGGUAg--UAAACUGCUUGGCa -3' miRNA: 3'- guCG---ACCAUggaAUUUGACGAACCGg -5' |
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23529 | 3' | -50 | NC_005260.1 | + | 1316 | 0.71 | 0.966524 |
Target: 5'- -uGCUGGUACCguucguaaagUUAAACaGCgUUGGUCa -3' miRNA: 3'- guCGACCAUGG----------AAUUUGaCG-AACCGG- -5' |
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23529 | 3' | -50 | NC_005260.1 | + | 41989 | 0.74 | 0.876434 |
Target: 5'- uCAGCUaGUGCCUgaggUAGACaUGCUUGGUa -3' miRNA: 3'- -GUCGAcCAUGGA----AUUUG-ACGAACCGg -5' |
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23529 | 3' | -50 | NC_005260.1 | + | 16455 | 1.13 | 0.007968 |
Target: 5'- uCAGCUGGUACCUUAAACUGCUUGGCCa -3' miRNA: 3'- -GUCGACCAUGGAAUUUGACGAACCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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